Improvement of cell lysis activity of immobilized lysozyme with reversibly soluble-insoluble polymer as carrier

Jyh Ping Chen; Yuh Cheng Chen

doi:10.1007/BF00222560

Improvement of cell lysis activity of immobilized lysozyme with reversibly soluble-insoluble polymer as carrier

Jyh Ping Chen^*
, Yuh Cheng Chen

^*Corresponding author for this work

Department of Chemical and Materials Engineering

National Cheng Kung University

Research output: Contribution to journal › Journal Article › peer-review

16 Scopus citations

Abstract

Hen egg white lysozyme was immobilized by covalent binding to a polymer showing reversibly soluble-insoluble characteristics with pH change. The retention of the specific activity of the immobilized enzyme can be as high as 41% of that of the free enzyme. The immobilized enzyme could be used in repeated batch lysis of M. lysodeikticus cells and to enhance the release of intracellular proteins 1.4 folds when compared with batch operation.

Original language	English
Pages (from-to)	749-754
Number of pages	6
Journal	Biotechnology Techniques
Volume	10
Issue number	10
DOIs	https://doi.org/10.1007/BF00222560
State	Published - 1996

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@article{1e2a5631945e4c84b19fb449a4126318,

title = "Improvement of cell lysis activity of immobilized lysozyme with reversibly soluble-insoluble polymer as carrier",

abstract = "Hen egg white lysozyme was immobilized by covalent binding to a polymer showing reversibly soluble-insoluble characteristics with pH change. The retention of the specific activity of the immobilized enzyme can be as high as 41\% of that of the free enzyme. The immobilized enzyme could be used in repeated batch lysis of M. lysodeikticus cells and to enhance the release of intracellular proteins 1.4 folds when compared with batch operation.",

author = "Chen, \{Jyh Ping\} and Chen, \{Yuh Cheng\}",

year = "1996",

doi = "10.1007/BF00222560",

language = "英语",

volume = "10",

pages = "749--754",

journal = "Biotechnology Techniques",

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T1 - Improvement of cell lysis activity of immobilized lysozyme with reversibly soluble-insoluble polymer as carrier

AU - Chen, Jyh Ping

AU - Chen, Yuh Cheng

PY - 1996

Y1 - 1996

N2 - Hen egg white lysozyme was immobilized by covalent binding to a polymer showing reversibly soluble-insoluble characteristics with pH change. The retention of the specific activity of the immobilized enzyme can be as high as 41% of that of the free enzyme. The immobilized enzyme could be used in repeated batch lysis of M. lysodeikticus cells and to enhance the release of intracellular proteins 1.4 folds when compared with batch operation.

AB - Hen egg white lysozyme was immobilized by covalent binding to a polymer showing reversibly soluble-insoluble characteristics with pH change. The retention of the specific activity of the immobilized enzyme can be as high as 41% of that of the free enzyme. The immobilized enzyme could be used in repeated batch lysis of M. lysodeikticus cells and to enhance the release of intracellular proteins 1.4 folds when compared with batch operation.

UR - https://www.scopus.com/pages/publications/0030470996

U2 - 10.1007/BF00222560

DO - 10.1007/BF00222560

M3 - 文章

AN - SCOPUS:0030470996

SN - 0951-208X

VL - 10

SP - 749

EP - 754

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 10

ER -

Improvement of cell lysis activity of immobilized lysozyme with reversibly soluble-insoluble polymer as carrier

Abstract

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