In vitro anti-influenza virus activity of synthetic humate analogues derived from protocatechuic acid

F. J. Lu, S. N. Tseng, M. L. Li, S. R. Shih*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

23 Scopus citations

Abstract

Two humic-like substances, the oxidative polymer of protocatechuic acid (OP-PCA) and humic acid inhibit the in vitro replication of influenza virus A/WSN/33 (H1N1) in Madin-Darby canine kidney (MDCK) cells at concentrations of no cytotoxicity. The 50% inhibitory concentration (IC50) for OP-PCA was 6.59 ± 1.02 μg/ml when the compound was added at the stage of viral adsorption. When OP-PCA was added after virus adsorption, the IC50 was 53.27 ± 8.12 μg/ml. The IC50 for humic acid was 48.61 ± 7.32 μg/ml and 55.27 ± 5.46 μg/ml respectively when the compound was added at the stage of viral adsorption or post-adsorption. In spite of structural resemblance of these two compounds, they exhibit different actions of anti-flu. The OP-PCA inhibits virus-induced hemagglutination and low pH-induced cell-cell fusion. Humic acid inhibits the endonuclease activity of viral RNA polymerase. The monomer of PCA shows no inhibition on influenza virus replication.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalArchives of Virology
Volume147
Issue number2
DOIs
StatePublished - 2002

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