In vitro hepatocyte-activity enhancement via a lobule-mimetic engineered liver tissue lab chip

Chung Kuang Chin, Rong Jhe Chen, Chien Yu Fu, Hwan You Chang, Cheng Hsien Liu*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

A liver labchip integrating the DEP-based cell patterning technique and the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement is demonstrated and reported in this paper. DEP forces are utilized as the cell-patterning mechanism to manipulate heterogeneous cells for mimicking the inherent hepatic morphology and increasing the cell-cell interaction. An ethoxyresorufin-O-dealkylase assay was performed to study the liver function enhancement of the engineered liver tissues. Experiment results show that the P450-1A1 enzyme activity of engineered liver tissues are 68.41% enhanced comparing with non-patterned liver cells.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages635-637
Number of pages3
StatePublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 03 10 201007 10 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period03/10/1007/10/10

Keywords

  • Cell patterning
  • Dielectrophoresis
  • Engineered tissue
  • Liver labchip

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