In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

Chen Wei Wu; Shilpa Sivashankar; Srinivasu Valagerahally Puttaswamy; Hui Ling Lin; Kuo Wei Chang; Chau Ting Yeh; Cheng Hsien Liu

doi:10.1109/NEMS.2012.6196740

In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

Chen Wei Wu^*, Shilpa Sivashankar, Srinivasu Valagerahally Puttaswamy, Hui Ling Lin, Kuo Wei Chang, Chau Ting Yeh, Cheng Hsien Liu

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

We report a Poly methyl methacrylate (PMMA) bioreactor in which hepatitis B virus (HBV) infected transgenic mice liver tissue has been cultured for long term with the continuous flow of culture medium, surrounded by mesothelial cells to provide 3-dimensional culturing conditions with adequate nutrient exchange. PDMS membrane was fabricated and a layer of mesothelial cells were cultured on it. Further this membrane was deformed and positioned on the microchannel by suction through the holes at the bottom of the channel. Liver tissue of transgenic mice was introduced on PDMS membrane. The experimental results proved that the proposed bioreactor portrays the in-vivo conditions better, with substantial improvement of liver-specific functions such as sufficient antigen expression, better structural integrity when compared to conventional static culture method. The in-vitro culture period of sliced liver tissue in our bioreactor was extended for 9 days to facilitate drug screening applications.

Original language	English
Title of host publication	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages	133-136
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2012.6196740
State	Published - 2012
Externally published	Yes
Event	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan Duration: 05 03 2012 → 08 03 2012

Publication series

Name	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Conference

Conference	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Country/Territory	Japan
City	Kyoto
Period	05/03/12 → 08/03/12

Keywords

Bioreactor
Hydrodynamic Flow
Tissue Culture
Transgenic Mice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/NEMS.2012.6196740

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Wu, C. W., Sivashankar, S., Valagerahally Puttaswamy, S., Lin, H. L., Chang, K. W., Yeh, C. T., & Liu, C. H. (2012). In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 133-136). Article 6196740 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196740

Wu, Chen Wei ; Sivashankar, Shilpa ; Valagerahally Puttaswamy, Srinivasu et al. / In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. pp. 133-136 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

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title = "In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor",

abstract = "We report a Poly methyl methacrylate (PMMA) bioreactor in which hepatitis B virus (HBV) infected transgenic mice liver tissue has been cultured for long term with the continuous flow of culture medium, surrounded by mesothelial cells to provide 3-dimensional culturing conditions with adequate nutrient exchange. PDMS membrane was fabricated and a layer of mesothelial cells were cultured on it. Further this membrane was deformed and positioned on the microchannel by suction through the holes at the bottom of the channel. Liver tissue of transgenic mice was introduced on PDMS membrane. The experimental results proved that the proposed bioreactor portrays the in-vivo conditions better, with substantial improvement of liver-specific functions such as sufficient antigen expression, better structural integrity when compared to conventional static culture method. The in-vitro culture period of sliced liver tissue in our bioreactor was extended for 9 days to facilitate drug screening applications.",

keywords = "Bioreactor, Hydrodynamic Flow, Tissue Culture, Transgenic Mice",

author = "Wu, {Chen Wei} and Shilpa Sivashankar and {Valagerahally Puttaswamy}, Srinivasu and Lin, {Hui Ling} and Chang, {Kuo Wei} and Yeh, {Chau Ting} and Liu, {Cheng Hsien}",

year = "2012",

doi = "10.1109/NEMS.2012.6196740",

language = "英语",

isbn = "9781467311243",

series = "2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012",

pages = "133--136",

booktitle = "2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012",

note = "7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 ; Conference date: 05-03-2012 Through 08-03-2012",

}

Wu, CW, Sivashankar, S, Valagerahally Puttaswamy, S, Lin, HL, Chang, KW, Yeh, CT & Liu, CH 2012, In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor. in 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012., 6196740, 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, pp. 133-136, 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, Kyoto, Japan, 05/03/12. https://doi.org/10.1109/NEMS.2012.6196740

In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor. / Wu, Chen Wei; Sivashankar, Shilpa; Valagerahally Puttaswamy, Srinivasu et al.
2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 133-136 6196740 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

AU - Wu, Chen Wei

AU - Sivashankar, Shilpa

AU - Valagerahally Puttaswamy, Srinivasu

AU - Lin, Hui Ling

AU - Chang, Kuo Wei

AU - Yeh, Chau Ting

AU - Liu, Cheng Hsien

PY - 2012

Y1 - 2012

N2 - We report a Poly methyl methacrylate (PMMA) bioreactor in which hepatitis B virus (HBV) infected transgenic mice liver tissue has been cultured for long term with the continuous flow of culture medium, surrounded by mesothelial cells to provide 3-dimensional culturing conditions with adequate nutrient exchange. PDMS membrane was fabricated and a layer of mesothelial cells were cultured on it. Further this membrane was deformed and positioned on the microchannel by suction through the holes at the bottom of the channel. Liver tissue of transgenic mice was introduced on PDMS membrane. The experimental results proved that the proposed bioreactor portrays the in-vivo conditions better, with substantial improvement of liver-specific functions such as sufficient antigen expression, better structural integrity when compared to conventional static culture method. The in-vitro culture period of sliced liver tissue in our bioreactor was extended for 9 days to facilitate drug screening applications.

AB - We report a Poly methyl methacrylate (PMMA) bioreactor in which hepatitis B virus (HBV) infected transgenic mice liver tissue has been cultured for long term with the continuous flow of culture medium, surrounded by mesothelial cells to provide 3-dimensional culturing conditions with adequate nutrient exchange. PDMS membrane was fabricated and a layer of mesothelial cells were cultured on it. Further this membrane was deformed and positioned on the microchannel by suction through the holes at the bottom of the channel. Liver tissue of transgenic mice was introduced on PDMS membrane. The experimental results proved that the proposed bioreactor portrays the in-vivo conditions better, with substantial improvement of liver-specific functions such as sufficient antigen expression, better structural integrity when compared to conventional static culture method. The in-vitro culture period of sliced liver tissue in our bioreactor was extended for 9 days to facilitate drug screening applications.

KW - Bioreactor

KW - Hydrodynamic Flow

KW - Tissue Culture

KW - Transgenic Mice

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M3 - 会议稿件

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SN - 9781467311243

T3 - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

SP - 133

EP - 136

BT - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

T2 - 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Y2 - 5 March 2012 through 8 March 2012

ER -

Wu CW, Sivashankar S, Valagerahally Puttaswamy S, Lin HL, Chang KW, Yeh CT et al. In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 133-136. 6196740. (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). doi: 10.1109/NEMS.2012.6196740

In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

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Keywords

UN SDGs

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