Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation

Shao Chun Wu; Pao Jen Kuo; Cheng Shyuan Rau; Lien Hung Huang; Chia Wei Lin; Yi Chan Wu; Chia Jung Wu; Chia Wen Tsai; Ting Min Hsieh; Hang Tsung Liu; Chun Ying Huang; Ching Hua Hsieh

doi:10.3390/ijms22168877

Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation

Shao Chun Wu, Pao Jen Kuo, Cheng Shyuan Rau, Lien Hung Huang, Chia Wei Lin, Yi Chan Wu, Chia Jung Wu, Chia Wen Tsai, Ting Min Hsieh, Hang Tsung Liu, Chun Ying Huang^*, Ching Hua Hsieh^*

^*Corresponding author for this work

Graduate Institute of Clinical Medical Sciences

Research output: Contribution to journal › Journal Article › peer-review

16 Scopus citations

Abstract

Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing. However, in clinical settings, wounds may be infected by various bacteria or pathogens. We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs). ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h. The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs. RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo. An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo. In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.

Original language	English
Article number	8877
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	16
DOIs	https://doi.org/10.3390/ijms22168877
State	Published - 02 08 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Activating protein 1
Adipose-derived stem cells
Angiogenesis
CAMP response element binding protein
Endothelial cell
Exosome
Interleukin-8
Lipopolysaccharide
Nuclear factor-κB
Proteomic analysis

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Wu, S. C., Kuo, P. J., Rau, C. S., Huang, L. H., Lin, C. W., Wu, Y. C., Wu, C. J., Tsai, C. W., Hsieh, T. M., Liu, H. T., Huang, C. Y., & Hsieh, C. H. (2021). Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation. International Journal of Molecular Sciences, 22(16), Article 8877. https://doi.org/10.3390/ijms22168877

@article{e6e175fa6dc9426bbc35c82a64cc72a5,

title = "Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation",

abstract = "Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing. However, in clinical settings, wounds may be infected by various bacteria or pathogens. We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs). ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h. The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs. RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo. An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo. In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.",

keywords = "Activating protein 1, Adipose-derived stem cells, Angiogenesis, CAMP response element binding protein, Endothelial cell, Exosome, Interleukin-8, Lipopolysaccharide, Nuclear factor-κB, Proteomic analysis",

author = "Wu, {Shao Chun} and Kuo, {Pao Jen} and Rau, {Cheng Shyuan} and Huang, {Lien Hung} and Lin, {Chia Wei} and Wu, {Yi Chan} and Wu, {Chia Jung} and Tsai, {Chia Wen} and Hsieh, {Ting Min} and Liu, {Hang Tsung} and Huang, {Chun Ying} and Hsieh, {Ching Hua}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = aug,

day = "2",

doi = "10.3390/ijms22168877",

language = "英语",

volume = "22",

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TY - JOUR

T1 - Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation

AU - Wu, Shao Chun

AU - Kuo, Pao Jen

AU - Rau, Cheng Shyuan

AU - Huang, Lien Hung

AU - Lin, Chia Wei

AU - Wu, Yi Chan

AU - Wu, Chia Jung

AU - Tsai, Chia Wen

AU - Hsieh, Ting Min

AU - Liu, Hang Tsung

AU - Huang, Chun Ying

AU - Hsieh, Ching Hua

PY - 2021/8/2

Y1 - 2021/8/2

N2 - Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing. However, in clinical settings, wounds may be infected by various bacteria or pathogens. We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs). ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h. The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs. RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo. An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo. In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.

AB - Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing. However, in clinical settings, wounds may be infected by various bacteria or pathogens. We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs). ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h. The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs. RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo. An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo. In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.

KW - Activating protein 1

KW - Adipose-derived stem cells

KW - Angiogenesis

KW - CAMP response element binding protein

KW - Endothelial cell

KW - Exosome

KW - Interleukin-8

KW - Lipopolysaccharide

KW - Nuclear factor-κB

KW - Proteomic analysis

UR - http://www.scopus.com/inward/record.url?scp=85112768129&partnerID=8YFLogxK

U2 - 10.3390/ijms22168877

DO - 10.3390/ijms22168877

M3 - 文章

C2 - 34445582

AN - SCOPUS:85112768129

SN - 1661-6596

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 16

M1 - 8877

ER -

Increased angiogenesis by exosomes secreted by adipose-derived stem cells upon lipopolysaccharide stimulation

Abstract

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Keywords

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