Increased mdr gene expression and decreased drug accumulation in a human colonic cancer cell line resistant to hydrophobic drug.

Chuck C.-K. Chao, CM Ma, PW Cheng, S Lin-Chao

Research output: Contribution to journalJournal Article peer-review

Abstract

An adriamycin-resistant human colonic cancer cell line was characterized. This clone exhibits the classical multidrug resistance (MDR) phenotype, being cross-resistant to hydrophobic drugs such as colchicine, and vinblastine. In contrast, this clone shows a normal response to DNA-damaging agents. The appearance of MDR in these cells was linked to a decreased accumulation of the drug [3H]colchicine as compared to the drug-sensitive cells. This MDR line expressed 80-100 fold increased levels of the specific 4.5-kb mdr mRNA, and a gene amplification. Our results indicate that MDR in human colonic cancer cells can result from increased expression of at least one member of the mdr gene family.
Original languageAmerican English
Pages (from-to)842-849
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
StatePublished - 1990

Keywords

  • Antineoplastic Agents/pharmacology
  • Base Sequence
  • Biological Transport
  • Blotting, Northern
  • Blotting, Southern
  • Cell Line
  • Cisplatin/pharmacology
  • Colchicine/metabolism
  • Colchicine/pharmacology
  • Colonic Neoplasms
  • DNA, Neoplasm/genetics
  • DNA, Neoplasm/isolation & purification
  • Doxorubicin/pharmacology
  • Drug Resistance/genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kinetics
  • Mitomycin
  • Mitomycins/pharmacology
  • Puromycin/pharmacology
  • RNA, Messenger/genetics
  • Vinblastine/pharmacology

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