Abstract
An adriamycin-resistant human colonic cancer cell line was characterized. This clone exhibits the classical multidrug resistance (MDR) phenotype, being cross-resistant to hydrophobic drugs such as colchicine, and vinblastine. In contrast, this clone shows a normal response to DNA-damaging agents. The appearance of MDR in these cells was linked to a decreased accumulation of the drug [3H]colchicine as compared to the drug-sensitive cells. This MDR line expressed 80-100 fold increased levels of the specific 4.5-kb mdr mRNA, and a gene amplification. Our results indicate that MDR in human colonic cancer cells can result from increased expression of at least one member of the mdr gene family.
| Original language | American English |
|---|---|
| Pages (from-to) | 842-849 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 172 |
| Issue number | 2 |
| State | Published - 1990 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Antineoplastic Agents/pharmacology
- Base Sequence
- Biological Transport
- Blotting, Northern
- Blotting, Southern
- Cell Line
- Cisplatin/pharmacology
- Colchicine/metabolism
- Colchicine/pharmacology
- Colonic Neoplasms
- DNA, Neoplasm/genetics
- DNA, Neoplasm/isolation & purification
- Doxorubicin/pharmacology
- Drug Resistance/genetics
- Gene Expression Regulation, Neoplastic
- Humans
- Kinetics
- Mitomycin
- Mitomycins/pharmacology
- Puromycin/pharmacology
- RNA, Messenger/genetics
- Vinblastine/pharmacology
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