Influence of hypothermic conditions on primary porcine hepatocyte-entrapped hollow fiber bioreactors

Chi Chang Lin; Kuo Chen Hung; Tai Horng Young; Yaw Sen Chen; Chee Chien Yong; Eiji Kobayashi; Chien Hung Wu; Chin Hsiung Yang; Chao Long Chen; Chih Chi Wang

doi:10.1016/j.bej.2005.02.030

Influence of hypothermic conditions on primary porcine hepatocyte-entrapped hollow fiber bioreactors

Chi Chang Lin, Kuo Chen Hung, Tai Horng Young, Yaw Sen Chen, Chee Chien Yong, Eiji Kobayashi, Chien Hung Wu, Chin Hsiung Yang, Chao Long Chen, Chih Chi Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

1 Scopus citations

Abstract

Transportation of bioartificial liver (BAL) device with viable cells and higher metabolic functions are necessary. The aim of this study is to evaluate hypothermic (4 °C) influence on the performance of hepatocyte-entrapped hollow fiber bioreactors, as well as the effects on cell function with or without medium supplement. Oxygen consumptions were stable at average of 15-25 mmHg per cartridge during 8 h normothermic incubation after cold-perfusion and cold-non-perfusion. All groups showed increase in glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) level. Urea production and albumin synthesis were only slightly higher in cold-perfusion groups than that of the cold-non-perfusion groups and the control. There were no significant differences in the metabolic functions of bioreactors between each study groups during normothermic operation. Our study suggests that the use of a preliminary cold-storage step prior to normal culture condition or clinical therapy can prolong the transportation time without changing various functions of the BAL device.

Original language	English
Pages (from-to)	139-148
Number of pages	10
Journal	Biochemical Engineering Journal
Volume	29
Issue number	1-2
DOIs	https://doi.org/10.1016/j.bej.2005.02.030
State	Published - 01 04 2006
Externally published	Yes

Keywords

Bioartificial liver
Cold storage
Hypothermic
Porcine hepatocytes

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title = "Influence of hypothermic conditions on primary porcine hepatocyte-entrapped hollow fiber bioreactors",

abstract = "Transportation of bioartificial liver (BAL) device with viable cells and higher metabolic functions are necessary. The aim of this study is to evaluate hypothermic (4 °C) influence on the performance of hepatocyte-entrapped hollow fiber bioreactors, as well as the effects on cell function with or without medium supplement. Oxygen consumptions were stable at average of 15-25 mmHg per cartridge during 8 h normothermic incubation after cold-perfusion and cold-non-perfusion. All groups showed increase in glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) level. Urea production and albumin synthesis were only slightly higher in cold-perfusion groups than that of the cold-non-perfusion groups and the control. There were no significant differences in the metabolic functions of bioreactors between each study groups during normothermic operation. Our study suggests that the use of a preliminary cold-storage step prior to normal culture condition or clinical therapy can prolong the transportation time without changing various functions of the BAL device.",

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author = "Lin, {Chi Chang} and Hung, {Kuo Chen} and Young, {Tai Horng} and Chen, {Yaw Sen} and Yong, {Chee Chien} and Eiji Kobayashi and Wu, {Chien Hung} and Yang, {Chin Hsiung} and Chen, {Chao Long} and Wang, {Chih Chi}",

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doi = "10.1016/j.bej.2005.02.030",

language = "英语",

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AU - Lin, Chi Chang

AU - Hung, Kuo Chen

AU - Young, Tai Horng

AU - Chen, Yaw Sen

AU - Yong, Chee Chien

AU - Kobayashi, Eiji

AU - Wu, Chien Hung

AU - Yang, Chin Hsiung

AU - Chen, Chao Long

AU - Wang, Chih Chi

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Transportation of bioartificial liver (BAL) device with viable cells and higher metabolic functions are necessary. The aim of this study is to evaluate hypothermic (4 °C) influence on the performance of hepatocyte-entrapped hollow fiber bioreactors, as well as the effects on cell function with or without medium supplement. Oxygen consumptions were stable at average of 15-25 mmHg per cartridge during 8 h normothermic incubation after cold-perfusion and cold-non-perfusion. All groups showed increase in glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) level. Urea production and albumin synthesis were only slightly higher in cold-perfusion groups than that of the cold-non-perfusion groups and the control. There were no significant differences in the metabolic functions of bioreactors between each study groups during normothermic operation. Our study suggests that the use of a preliminary cold-storage step prior to normal culture condition or clinical therapy can prolong the transportation time without changing various functions of the BAL device.

AB - Transportation of bioartificial liver (BAL) device with viable cells and higher metabolic functions are necessary. The aim of this study is to evaluate hypothermic (4 °C) influence on the performance of hepatocyte-entrapped hollow fiber bioreactors, as well as the effects on cell function with or without medium supplement. Oxygen consumptions were stable at average of 15-25 mmHg per cartridge during 8 h normothermic incubation after cold-perfusion and cold-non-perfusion. All groups showed increase in glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) level. Urea production and albumin synthesis were only slightly higher in cold-perfusion groups than that of the cold-non-perfusion groups and the control. There were no significant differences in the metabolic functions of bioreactors between each study groups during normothermic operation. Our study suggests that the use of a preliminary cold-storage step prior to normal culture condition or clinical therapy can prolong the transportation time without changing various functions of the BAL device.

KW - Bioartificial liver

KW - Cold storage

KW - Hypothermic

KW - Porcine hepatocytes

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Influence of hypothermic conditions on primary porcine hepatocyte-entrapped hollow fiber bioreactors

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Keywords

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