Influenza A Virus PB1-F2 Gene in Recent Taiwanese Isolates

Guang Wu Chen; Ching Chun Yang; Kuo Chien Tsao; Chung Guei Huang; Li Ang Lee; Wen Zhi Yang; Ya Ling Huang; Tzou Yien Lin; Shin Ru Shih

doi:10.3201/eid1004.030412

Influenza A Virus PB1-F2 Gene in Recent Taiwanese Isolates

Guang Wu Chen, Ching Chun Yang, Kuo Chien Tsao, Chung Guei Huang, Li Ang Lee, Wen Zhi Yang, Ya Ling Huang, Tzou Yien Lin, Shin Ru Shih^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

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Abstract

Influenza A virus contains eight RNA segments and encodes 10 viral proteins. However, an 11th protein, called PB1-F2, was found in A/Puerto Rico/8/34 (H1N1). This novel protein is translated from an alternative open reading frame (ORF) in the PB1 gene. We analyzed the PB1 gene of 42 recent influenza A isolates in Taiwan, including 24 H1N1 and 18 H3N2 strains. One H1N1 isolate and 17 H3N2 isolates contained the entire PB1-F2 ORF of 90 residues, three amino acids (aa) longer than the PB1-F2 of A/Puerto Rico/8/34 at the C terminal. The one remaining H3N2 isolate encoded a truncated PB1-F2 with 79 residues. The other 23 H1N1 isolates contained a truncated PB1-F2 of 57 aa. Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.

Original language	English
Pages (from-to)	630-636
Number of pages	7
Journal	Emerging Infectious Diseases
Volume	10
Issue number	4
DOIs	https://doi.org/10.3201/eid1004.030412
State	Published - 04 2004

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title = "Influenza A Virus PB1-F2 Gene in Recent Taiwanese Isolates",

abstract = "Influenza A virus contains eight RNA segments and encodes 10 viral proteins. However, an 11th protein, called PB1-F2, was found in A/Puerto Rico/8/34 (H1N1). This novel protein is translated from an alternative open reading frame (ORF) in the PB1 gene. We analyzed the PB1 gene of 42 recent influenza A isolates in Taiwan, including 24 H1N1 and 18 H3N2 strains. One H1N1 isolate and 17 H3N2 isolates contained the entire PB1-F2 ORF of 90 residues, three amino acids (aa) longer than the PB1-F2 of A/Puerto Rico/8/34 at the C terminal. The one remaining H3N2 isolate encoded a truncated PB1-F2 with 79 residues. The other 23 H1N1 isolates contained a truncated PB1-F2 of 57 aa. Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.",

author = "Chen, {Guang Wu} and Yang, {Ching Chun} and Tsao, {Kuo Chien} and Huang, {Chung Guei} and Lee, {Li Ang} and Yang, {Wen Zhi} and Huang, {Ya Ling} and Lin, {Tzou Yien} and Shih, {Shin Ru}",

year = "2004",

month = apr,

doi = "10.3201/eid1004.030412",

language = "英语",

volume = "10",

pages = "630--636",

journal = "Emerging Infectious Diseases",

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publisher = "Centers for Disease Control and Prevention (CDC)",

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T1 - Influenza A Virus PB1-F2 Gene in Recent Taiwanese Isolates

AU - Chen, Guang Wu

AU - Yang, Ching Chun

AU - Tsao, Kuo Chien

AU - Huang, Chung Guei

AU - Lee, Li Ang

AU - Yang, Wen Zhi

AU - Huang, Ya Ling

AU - Lin, Tzou Yien

AU - Shih, Shin Ru

PY - 2004/4

Y1 - 2004/4

N2 - Influenza A virus contains eight RNA segments and encodes 10 viral proteins. However, an 11th protein, called PB1-F2, was found in A/Puerto Rico/8/34 (H1N1). This novel protein is translated from an alternative open reading frame (ORF) in the PB1 gene. We analyzed the PB1 gene of 42 recent influenza A isolates in Taiwan, including 24 H1N1 and 18 H3N2 strains. One H1N1 isolate and 17 H3N2 isolates contained the entire PB1-F2 ORF of 90 residues, three amino acids (aa) longer than the PB1-F2 of A/Puerto Rico/8/34 at the C terminal. The one remaining H3N2 isolate encoded a truncated PB1-F2 with 79 residues. The other 23 H1N1 isolates contained a truncated PB1-F2 of 57 aa. Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.

AB - Influenza A virus contains eight RNA segments and encodes 10 viral proteins. However, an 11th protein, called PB1-F2, was found in A/Puerto Rico/8/34 (H1N1). This novel protein is translated from an alternative open reading frame (ORF) in the PB1 gene. We analyzed the PB1 gene of 42 recent influenza A isolates in Taiwan, including 24 H1N1 and 18 H3N2 strains. One H1N1 isolate and 17 H3N2 isolates contained the entire PB1-F2 ORF of 90 residues, three amino acids (aa) longer than the PB1-F2 of A/Puerto Rico/8/34 at the C terminal. The one remaining H3N2 isolate encoded a truncated PB1-F2 with 79 residues. The other 23 H1N1 isolates contained a truncated PB1-F2 of 57 aa. Phylogenetic analysis of both the HA and the PB1 genes showed that they shared similar clustering of these Taiwanese isolates, suggesting that no obvious reassortment occurred between the two genomic segments.

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U2 - 10.3201/eid1004.030412

DO - 10.3201/eid1004.030412

M3 - 文章

C2 - 15200852

AN - SCOPUS:1842588700

SN - 1080-6040

VL - 10

SP - 630

EP - 636

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 4

ER -

Influenza A Virus PB1-F2 Gene in Recent Taiwanese Isolates

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