Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device

Pallavi Shinde, Srabani Kar, Mohan Loganathan, Hwan You Chang, Fan Gang Tseng, Moeto Nagai, Tuhin Subhra Santra*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

30 Scopus citations


We report infrared (IR) pulse laser-activated highly efficient parallel intracellular delivery by using an array of titanium microdish (TMD) device. Upon IR laser pulse irradiation, a two-dimensional array of TMD device generated photothermal cavitation bubbles to disrupt the cell membrane surface and create transient membrane pores to deliver biomolecules into cells by a simple diffusion process. We successfully delivered the dyes and different sizes of dextran in different cell types with variations of laser pulses. Our platform has the ability to transfect more than a million cells in a parallel fashion within a minute. The best results were achieved for SiHa cells with a delivery efficiency of 96% and a cell viability of around 98% for propidium iodide dye using 600 pulses, whereas a delivery efficiency of 98% and a cell viability of 100% were obtained for dextran 3000 MW delivery using 700 pulses. For dextran 10,000 MW, the delivery efficiency was 92% and the cell viability was 98%, respectively. The device is compact, easy-to-use, and potentially applicable for cellular therapy and diagnostic purposes.

Original languageEnglish
Pages (from-to)5645-5652
Number of pages8
JournalACS Biomaterials Science and Engineering
Issue number10
StatePublished - 12 10 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2020 American Chemical Society.


  • Ti microdish
  • cell viability
  • delivery efficiency
  • infrared light pulse irradiation
  • parallel intracellular delivery
  • transient heating


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