Inhibition of aldosterone production by testosterone in male rats

Mei Mei Kau, Ming Jae Lo, Shyi Wu Wang, Shiow Chwen Tsai, Jiann Jong Chen, Yu Chung Chiao, Jiun Yih Yeh, Ho Lin, Andrew Yau Chik Shum, Victor S. Fang, Low Tone Ho, Paulus S. Wang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

44 Scopus citations


In vivo and in vitro experiments were designed to assess the effect of testosterone on aldosterone secretion in male rats. Orchidectomized rats were injected subcutaneously with oil or testosterone propionate ([TP] 2 mg/kg) for 7 days. Intact rats were injected with oil only. The results indicate that the plasma aldosterone level was higher in orchidectomized versus intact and TP-replaced rats. In the in vitro study, testosterone caused a marked decrease of aldosterone secretion by zona glomerulosa (ZG) cells, but failed to alter the accumulation of intracellular adenosine 3',5'-cyclic monophosphate (cAMP). Testosterone significantly decreased the corticotropin (ACTH)-stimulated production of aldosterone and accumulation of cAMP in rat ZG cells. The conversion of corticosterone to aldosterone and of 25-OH- cholesterol to pregnenolone, as well as angiotensin II (ANG II)-stimulated production of aldosterone, were decreased by testosterone. These results suggest that testosterone inhibits the basal and ANG II- and ACTH-stimulated release of aldosterone, via inhibition of aldosterone synthase activity and cytochrome P-450 side-chain cleavage (P450scc) activity, and ACTH-stimulated cAMP accumulation in rat ZG cells.

Original languageEnglish
Pages (from-to)1108-1114
Number of pages7
JournalMetabolism: Clinical and Experimental
Issue number9
StatePublished - 1999


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