Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis

Chien Neng Kuo; Lin Cheng Yang; Cheng Ta Yang; Chien Hsiung Lai; Miao Fen Chen; Chung Yi Chen; Chi Hung Chen; Pei Chang Wu; Hsi Kung Kou; Yung Jen Chen; Chia Hui Hung; Chong Bin Tsai

doi:10.1016/j.exer.2009.06.021

Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis

Chien Neng Kuo, Lin Cheng Yang, Cheng Ta Yang, Chien Hsiung Lai, Miao Fen Chen, Chung Yi Chen, Chi Hung Chen, Pei Chang Wu, Hsi Kung Kou, Yung Jen Chen, Chia Hui Hung^*, Chong Bin Tsai

^*Corresponding author for this work

School of Traditional Chinese Medicine

Research output: Contribution to journal › Journal Article › peer-review

24 Scopus citations

Abstract

The use of Synthetic Amphiphile INTeraction-18 (SAINT-18) carrying plasmid pigment epithelium-derived factor (p-PEDF) as an anti-angiogenesis strategy to treat corneal neovascularization in a rat model was evaluated. Four partially dried forms (Group A: 0 μg, B: 0.1 μg, C: 1 μg, D: 10 μg) of a p-PEDF-SAINT-18 were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at the temporal side. The 1 μg of plasmid-basic fibroblast growth factor--SAINT-18 (p-bFGF-SAINT-18) (1 μg) was prepared and implanted into the rat corneal stroma 1.5 mm from the limbus on the same side. Inhibition of neovascularization was observed and quantified from day 1 to day 60. PEDF (50-kDa) and bFGF (18-kDa) protein expression were analyzed by biomicroscopic examination, Western blot analysis, and immunohistochemistry. Gene expression in corneal and conjunctival tissue was observed as early as 3 days after gene transfer and stably lasted for over 3 months with minimal immune reaction. Subconjunctival injection of a highly efficient p-PEDF-SAINT-18 successfully inhibited corneal neovascularization. Successful gene expression of bFGF, PEDF and a mild immune response of HLA-DR were shown by immunohistochemistry staining. We concluded that SAINT-18 was capable of directly delivering genes to the ocular surface by way of subconjunctival injection, and delivered sustained, high levels of gene expression in vivo to inhibit angiogenesis. Crown

Original language	English
Pages (from-to)	678-685
Number of pages	8
Journal	Experimental Eye Research
Volume	89
Issue number	5
DOIs	https://doi.org/10.1016/j.exer.2009.06.021
State	Published - 11 2009

Keywords

SAINT-18 (synthetic amphiphile interaction-18), 1-methyl-4-(cis-9-dioleyl)methylpyridinium-chloride
angiogenesis
cornea
fibroblast growth factor
nonviral vector
pigment epithelium-derived factor

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10.1016/j.exer.2009.06.021

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Kuo, C. N., Yang, L. C., Yang, C. T., Lai, C. H., Chen, M. F., Chen, C. Y., Chen, C. H., Wu, P. C., Kou, H. K., Chen, Y. J., Hung, C. H., & Tsai, C. B. (2009). Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis. Experimental Eye Research, 89(5), 678-685. https://doi.org/10.1016/j.exer.2009.06.021

@article{1314efc8a19743d69e5df6542c12cc84,

title = "Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis",

abstract = "The use of Synthetic Amphiphile INTeraction-18 (SAINT-18) carrying plasmid pigment epithelium-derived factor (p-PEDF) as an anti-angiogenesis strategy to treat corneal neovascularization in a rat model was evaluated. Four partially dried forms (Group A: 0 μg, B: 0.1 μg, C: 1 μg, D: 10 μg) of a p-PEDF-SAINT-18 were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at the temporal side. The 1 μg of plasmid-basic fibroblast growth factor--SAINT-18 (p-bFGF-SAINT-18) (1 μg) was prepared and implanted into the rat corneal stroma 1.5 mm from the limbus on the same side. Inhibition of neovascularization was observed and quantified from day 1 to day 60. PEDF (50-kDa) and bFGF (18-kDa) protein expression were analyzed by biomicroscopic examination, Western blot analysis, and immunohistochemistry. Gene expression in corneal and conjunctival tissue was observed as early as 3 days after gene transfer and stably lasted for over 3 months with minimal immune reaction. Subconjunctival injection of a highly efficient p-PEDF-SAINT-18 successfully inhibited corneal neovascularization. Successful gene expression of bFGF, PEDF and a mild immune response of HLA-DR were shown by immunohistochemistry staining. We concluded that SAINT-18 was capable of directly delivering genes to the ocular surface by way of subconjunctival injection, and delivered sustained, high levels of gene expression in vivo to inhibit angiogenesis. Crown",

keywords = "SAINT-18 (synthetic amphiphile interaction-18), 1-methyl-4-(cis-9-dioleyl)methylpyridinium-chloride, angiogenesis, cornea, fibroblast growth factor, nonviral vector, pigment epithelium-derived factor",

author = "Kuo, {Chien Neng} and Yang, {Lin Cheng} and Yang, {Cheng Ta} and Lai, {Chien Hsiung} and Chen, {Miao Fen} and Chen, {Chung Yi} and Chen, {Chi Hung} and Wu, {Pei Chang} and Kou, {Hsi Kung} and Chen, {Yung Jen} and Hung, {Chia Hui} and Tsai, {Chong Bin}",

year = "2009",

month = nov,

doi = "10.1016/j.exer.2009.06.021",

language = "英语",

volume = "89",

pages = "678--685",

journal = "Experimental Eye Research",

issn = "0014-4835",

publisher = "Academic Press",

number = "5",

}

Kuo, CN, Yang, LC, Yang, CT, Lai, CH, Chen, MF, Chen, CY, Chen, CH, Wu, PC, Kou, HK, Chen, YJ, Hung, CH & Tsai, CB 2009, 'Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis', Experimental Eye Research, vol. 89, no. 5, pp. 678-685. https://doi.org/10.1016/j.exer.2009.06.021

Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis. / Kuo, Chien Neng; Yang, Lin Cheng; Yang, Cheng Ta et al.
In: Experimental Eye Research, Vol. 89, No. 5, 11.2009, p. 678-685.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis

AU - Kuo, Chien Neng

AU - Yang, Lin Cheng

AU - Yang, Cheng Ta

AU - Lai, Chien Hsiung

AU - Chen, Miao Fen

AU - Chen, Chung Yi

AU - Chen, Chi Hung

AU - Wu, Pei Chang

AU - Kou, Hsi Kung

AU - Chen, Yung Jen

AU - Hung, Chia Hui

AU - Tsai, Chong Bin

PY - 2009/11

Y1 - 2009/11

N2 - The use of Synthetic Amphiphile INTeraction-18 (SAINT-18) carrying plasmid pigment epithelium-derived factor (p-PEDF) as an anti-angiogenesis strategy to treat corneal neovascularization in a rat model was evaluated. Four partially dried forms (Group A: 0 μg, B: 0.1 μg, C: 1 μg, D: 10 μg) of a p-PEDF-SAINT-18 were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at the temporal side. The 1 μg of plasmid-basic fibroblast growth factor--SAINT-18 (p-bFGF-SAINT-18) (1 μg) was prepared and implanted into the rat corneal stroma 1.5 mm from the limbus on the same side. Inhibition of neovascularization was observed and quantified from day 1 to day 60. PEDF (50-kDa) and bFGF (18-kDa) protein expression were analyzed by biomicroscopic examination, Western blot analysis, and immunohistochemistry. Gene expression in corneal and conjunctival tissue was observed as early as 3 days after gene transfer and stably lasted for over 3 months with minimal immune reaction. Subconjunctival injection of a highly efficient p-PEDF-SAINT-18 successfully inhibited corneal neovascularization. Successful gene expression of bFGF, PEDF and a mild immune response of HLA-DR were shown by immunohistochemistry staining. We concluded that SAINT-18 was capable of directly delivering genes to the ocular surface by way of subconjunctival injection, and delivered sustained, high levels of gene expression in vivo to inhibit angiogenesis. Crown

AB - The use of Synthetic Amphiphile INTeraction-18 (SAINT-18) carrying plasmid pigment epithelium-derived factor (p-PEDF) as an anti-angiogenesis strategy to treat corneal neovascularization in a rat model was evaluated. Four partially dried forms (Group A: 0 μg, B: 0.1 μg, C: 1 μg, D: 10 μg) of a p-PEDF-SAINT-18 were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at the temporal side. The 1 μg of plasmid-basic fibroblast growth factor--SAINT-18 (p-bFGF-SAINT-18) (1 μg) was prepared and implanted into the rat corneal stroma 1.5 mm from the limbus on the same side. Inhibition of neovascularization was observed and quantified from day 1 to day 60. PEDF (50-kDa) and bFGF (18-kDa) protein expression were analyzed by biomicroscopic examination, Western blot analysis, and immunohistochemistry. Gene expression in corneal and conjunctival tissue was observed as early as 3 days after gene transfer and stably lasted for over 3 months with minimal immune reaction. Subconjunctival injection of a highly efficient p-PEDF-SAINT-18 successfully inhibited corneal neovascularization. Successful gene expression of bFGF, PEDF and a mild immune response of HLA-DR were shown by immunohistochemistry staining. We concluded that SAINT-18 was capable of directly delivering genes to the ocular surface by way of subconjunctival injection, and delivered sustained, high levels of gene expression in vivo to inhibit angiogenesis. Crown

KW - SAINT-18 (synthetic amphiphile interaction-18), 1-methyl-4-(cis-9-dioleyl)methylpyridinium-chloride

KW - angiogenesis

KW - cornea

KW - fibroblast growth factor

KW - nonviral vector

KW - pigment epithelium-derived factor

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U2 - 10.1016/j.exer.2009.06.021

DO - 10.1016/j.exer.2009.06.021

M3 - 文章

C2 - 19596319

AN - SCOPUS:70349459725

SN - 0014-4835

VL - 89

SP - 678

EP - 685

JO - Experimental Eye Research

JF - Experimental Eye Research

IS - 5

ER -

Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis

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Keywords

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