Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

Li Kwan Chang; Ta Tung Wei; Ya Fang Chiu; Chao Ping Tung; Jian Ying Chuang; Shang Kai Hung; Ching Li; Shih Tung Liu

doi:10.1016/S0006-291X(03)00067-6

Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

Li Kwan Chang
, Ta Tung Wei
, Ya Fang Chiu
, Chao Ping Tung
, Jian Ying Chuang
, Shang Kai Hung
, Ching Li
, Shih Tung Liu^*

^*Corresponding author for this work

Department of Microbiology and Immunology

Kaohsiung Medical University
Chang Gung University
Chung Shan Medical University

Research output: Contribution to journal › Journal Article › peer-review

117 Scopus citations

Abstract

(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.

Original language	English
Pages (from-to)	1062-1068
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	301
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(03)00067-6
State	Published - 21 02 2003

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title = "Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate",

abstract = "(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.",

author = "Chang, \{Li Kwan\} and Wei, \{Ta Tung\} and Chiu, \{Ya Fang\} and Tung, \{Chao Ping\} and Chuang, \{Jian Ying\} and Hung, \{Shang Kai\} and Ching Li and Liu, \{Shih Tung\}",

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T1 - Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

AU - Chang, Li Kwan

AU - Wei, Ta Tung

AU - Chiu, Ya Fang

AU - Tung, Chao Ping

AU - Chuang, Jian Ying

AU - Hung, Shang Kai

AU - Li, Ching

AU - Liu, Shih Tung

PY - 2003/2/21

Y1 - 2003/2/21

N2 - (-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.

AB - (-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.

UR - https://www.scopus.com/pages/publications/0037459049

U2 - 10.1016/S0006-291X(03)00067-6

DO - 10.1016/S0006-291X(03)00067-6

M3 - 文章

C2 - 12589821

AN - SCOPUS:0037459049

SN - 0006-291X

VL - 301

SP - 1062

EP - 1068

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

Abstract

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