Inhibition of Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells by Phyllanthus urinaria extracts

Chih Ho Lai, Shih Hua Fang, Yerra Koteswara Rao, Madamanchi Geethangili, Chih Hsin Tang, Ying Ju Lin, Chien Hui Hung, Wen Ching Wang, Yew Min Tzeng*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

52 Scopus citations

Abstract

Aim of the study: Helicobacter pylori is linked to a majority of peptic ulcers and to some types of gastric cancer, and its resistance to antibiotic treatment is now found worldwide. This study is aimed at evaluating the antimicrobial activity of Phyllanthus urinaria Linnea (Euphorbiaceae), chloroform (PUC) and methanol (PUM) extracts, and its eight isolates on H. pylori-infected human gastric epithelial AGS cells. Materials and methods: The in vitro anti-bacterial activity of P. urinaria chloroform (PUC) and methanol (PUM) extracts, and its eight isolates were determined. Additional experiments were also performed to know the PUC and PUM ability to inhibit the H. pylori adhesion to and invasion of AGS cells, in addition to the effect of PUC on NF-κB activity as well as IL-8 synthesis during H. pylori infection of AGS cells. Results: The results revealed that crude extracts PUC and PUM showed potent antimicrobial activity against H. pylori than pure isolates. On the other hand, in vitro H. pylori-infection model revealed that the inhibition of bacterial adhesion and invasion to AGS cells has dramatically reduced by treatment of extract PUC, while PUM has the same moderate effect. Furthermore, H. pylori-induced nuclear factor (NF)-κB activation, and the subsequent release of interleukin (IL)-8 in AGS cells were also inhibited by the extract PUC. Conclusions: These results open the possibility of considering P. urinaria a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future.

Original languageEnglish
Pages (from-to)522-526
Number of pages5
JournalJournal of Ethnopharmacology
Volume118
Issue number3
DOIs
StatePublished - 13 08 2008
Externally publishedYes

Keywords

  • Chloroform and methanol extracts
  • Helicobacter pylori infection
  • Human gastric epithelial cells
  • IL-8
  • Isolates
  • NF-k{cyrillic}B
  • Phyllanthus urinaria

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