TY - JOUR
T1 - Inhibition of NFκB activation with curcumin attenuates plasma inflammatory cytokines surge and cardiomyocytic apoptosis following cardiac ischemia/reperfusion
AU - Yeh, Chi Hsiao
AU - Chen, Tzu Ping
AU - Wu, Yi Cheng
AU - Lin, Yu Min
AU - Jing Lin, Pyng
PY - 2005/5/1
Y1 - 2005/5/1
N2 - Background. Following cardiopulmonary bypass (CPB) and cardiac global ischemia and reperfusion, pro-inflammatory cytokines are activated and cause cardiomyocytic injury. Nuclear factor (NF)-κB is involved in regulating inflammatory signal transduction. Curcumin inhibits NF-κB activation and blocks the inflammatory responses. We studied whether curcumin could decrease myocardial ischemia/reperfusion injury with cardioplegia during CPB and attenuate the appearance of apoptosis of cardiomyocytes. Materials and methods. Rabbits received normal saline (group 1) or curcumin (70 μm/kg, group 2; 100 μm/kg, group 3) injection 2 h before CPB. Total CPB was initiated and cold (4°C) antegrade intermittent crystalloid cardioplegia was delivered every 20 min for 60 min of cardiac arrest. Rabbits were weaned from CPB and reperfused for 4 h. Blood was sampled at various time points and then the reperfused hearts were harvested. Results. Postoperative elevation of plasma levels of interleukin (IL)-8 (14.5, 0.9, 2.9 times over baseline in groups 1-3, respectively, P < 0.05), IL-10 (201.1, 6.0, 14.9 times over baseline in groups 1-3, respectively, P < 0.05), TNF-α (9.4, 3.1, 3.9 times over baseline in groups 1-3, respectively, P < 0.05), and cardiac troponin I (141.2, 14.9, 15.0 times over baseline) significantly decreased in the curcumin groups. Appearance of apoptotic cardiomyocytes significantly decreased in the curcumin groups (5.69 ± 1.64, 1.51 ± 0.41, 2.43 ± 0.49 per 1000 nuclei in groups 1-3, respectively, P < 0.01). The activation of neutrophil in the myocardium, which was measured using myocardial myloperoxidase activity assay, was significantly attenuated in the curcumin group. There was a significant increase in apoptosis-related cleavage fragments of caspase-3 and poly-ADP-ribose polymerase in group 1 compared to the other groups. Conclusions. Curcumin, an inhibitor of NF-κB, ameliorated the surge of pro-inflammatory cytokines during CPB and decreased the occurrence of cardiomyocytic apoptosis after global cardiac ischemia/reperfusion injury.
AB - Background. Following cardiopulmonary bypass (CPB) and cardiac global ischemia and reperfusion, pro-inflammatory cytokines are activated and cause cardiomyocytic injury. Nuclear factor (NF)-κB is involved in regulating inflammatory signal transduction. Curcumin inhibits NF-κB activation and blocks the inflammatory responses. We studied whether curcumin could decrease myocardial ischemia/reperfusion injury with cardioplegia during CPB and attenuate the appearance of apoptosis of cardiomyocytes. Materials and methods. Rabbits received normal saline (group 1) or curcumin (70 μm/kg, group 2; 100 μm/kg, group 3) injection 2 h before CPB. Total CPB was initiated and cold (4°C) antegrade intermittent crystalloid cardioplegia was delivered every 20 min for 60 min of cardiac arrest. Rabbits were weaned from CPB and reperfused for 4 h. Blood was sampled at various time points and then the reperfused hearts were harvested. Results. Postoperative elevation of plasma levels of interleukin (IL)-8 (14.5, 0.9, 2.9 times over baseline in groups 1-3, respectively, P < 0.05), IL-10 (201.1, 6.0, 14.9 times over baseline in groups 1-3, respectively, P < 0.05), TNF-α (9.4, 3.1, 3.9 times over baseline in groups 1-3, respectively, P < 0.05), and cardiac troponin I (141.2, 14.9, 15.0 times over baseline) significantly decreased in the curcumin groups. Appearance of apoptotic cardiomyocytes significantly decreased in the curcumin groups (5.69 ± 1.64, 1.51 ± 0.41, 2.43 ± 0.49 per 1000 nuclei in groups 1-3, respectively, P < 0.01). The activation of neutrophil in the myocardium, which was measured using myocardial myloperoxidase activity assay, was significantly attenuated in the curcumin group. There was a significant increase in apoptosis-related cleavage fragments of caspase-3 and poly-ADP-ribose polymerase in group 1 compared to the other groups. Conclusions. Curcumin, an inhibitor of NF-κB, ameliorated the surge of pro-inflammatory cytokines during CPB and decreased the occurrence of cardiomyocytic apoptosis after global cardiac ischemia/reperfusion injury.
KW - Apoptosis
KW - Cardiopulmonary bypass
KW - Curcumin
KW - NFκB
UR - http://www.scopus.com/inward/record.url?scp=17144382445&partnerID=8YFLogxK
U2 - 10.1016/j.jss.2004.11.009
DO - 10.1016/j.jss.2004.11.009
M3 - 文章
C2 - 15836859
AN - SCOPUS:17144382445
SN - 0022-4804
VL - 125
SP - 109
EP - 116
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -