Inhibition of swarming and virulence factor expression in Proteus mirabilis by resveratrol

Won Bo Wang, Hsin Chih Lai, Po Ren Hsueh, Robin Y.Y. Chiou, Shwu Bin Lin, Shwu Jen Liaw*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

115 Scopus citations

Abstract

Resveratrol (3,5,4-trihydroxy-frans-stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. The effect of resveratrol on swarming and virulence factor expression of Proteus mirabilis, an important pathogen infecting the urinary tract, was determined on swarming agar plates with and without the compound. Bacteria harvested at different times were assayed for cell length and the production of flagella, haemolysin and urease. Resveratrol inhibited P. mirabilis swarming and virulence factor expression in a dose-dependent manner. Resveratrol significantly inhibited swarming at 15 μg ml-1, and completely inhibited swarming at 60 μg ml-1. Inhibition of swarming and virulence factor expression was mediated through RsbA, a His-containing phosphotransmitter of the bacterial two-component signalling system possibly involved in quorum sensing. Complementation of an rsbA-defective mutant with the rsbA gene restored its responsiveness to resveratrol. The compound also inhibited the ability of P. mirabilis to invade human urothelial cells. These findings suggest that resveratrol has potential to be developed as an antimicrobial agent against P. mirabilis infection.

Original languageEnglish
Pages (from-to)1313-1321
Number of pages9
JournalJournal of Medical Microbiology
Volume55
Issue number10
DOIs
StatePublished - 10 2006
Externally publishedYes

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