TY - JOUR
T1 - Inhibitory effect of Antrodia camphorata constituents on the Helicobacter pylori-associated gastric inflammation
AU - Geethangili, Madamanchi
AU - Fang, Shih Hua
AU - Lai, Chih Ho
AU - Rao, Yerra Koteswara
AU - Lien, Hsiu Man
AU - Tzeng, Yew Min
PY - 2010/3/1
Y1 - 2010/3/1
N2 - Helicobacter pylori infection plays a crucial role in the pathogenesis of peptic ulcer, and gastric cancer. Among the 13 chemical constituents isolated from fruiting bodies of Antrodia camphorata, methyl antcinate B (4), antcins K (10) and A (12) displayed potential anti-H. pylori activity with inhibition zones of 13, 12 and 10 mm, respectively, at a concentration of 0.2 mM. The isolates 4 and 10 exhibited a dose response inhibition of H. pylori adhesion and invasion to AGS cells in a range of concentrations between 0.005 and 0.02 mM, while 12 has moderate effect at relatively higher concentration. Furthermore, at these concentrations (0.005-0.02 mM) the isolates 4 and 10 also inhibited the H. pylori-induced nuclear factor (NF)-k{cyrillic}B activation, and the subsequent release of interleukin (IL)-8 in AGS cells. These results open the possibility of considering A. camphorata a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future.
AB - Helicobacter pylori infection plays a crucial role in the pathogenesis of peptic ulcer, and gastric cancer. Among the 13 chemical constituents isolated from fruiting bodies of Antrodia camphorata, methyl antcinate B (4), antcins K (10) and A (12) displayed potential anti-H. pylori activity with inhibition zones of 13, 12 and 10 mm, respectively, at a concentration of 0.2 mM. The isolates 4 and 10 exhibited a dose response inhibition of H. pylori adhesion and invasion to AGS cells in a range of concentrations between 0.005 and 0.02 mM, while 12 has moderate effect at relatively higher concentration. Furthermore, at these concentrations (0.005-0.02 mM) the isolates 4 and 10 also inhibited the H. pylori-induced nuclear factor (NF)-k{cyrillic}B activation, and the subsequent release of interleukin (IL)-8 in AGS cells. These results open the possibility of considering A. camphorata a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future.
KW - Antrodia camphorata
KW - Helicobacter pylori infection
KW - Human gastric epithelial cells
KW - IL-8
KW - Isolated constituents
KW - NF-k{cyrillic}B
UR - http://www.scopus.com/inward/record.url?scp=70349840923&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2009.06.006
DO - 10.1016/j.foodchem.2009.06.006
M3 - 文章
AN - SCOPUS:70349840923
SN - 0308-8146
VL - 119
SP - 149
EP - 153
JO - Food Chemistry
JF - Food Chemistry
IS - 1
ER -
