Initiation of RNA replication of cloned Taiwan-3 isolate of hepatitis delta virus genotype II in cultured cells

Feng Ming Lin, Chuan Mo Lee, Tzu Chi Wang, Mei Chao*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

Hepatitis delta virus (HDV) genotype II is the predominant genotype in Taiwan and is associated with less progressive disease than genotype I. Although the Taiwan-3 (T3) clone was the first genotype II HDV isolated in Taiwan, its replication in cultured cells has not previously been established. Here, we demonstrate that cloned T3 HDV is capable of replicating in cultured cells. Furthermore, we show that: (1) the replication level of T3 clones is 100-fold lower than that of a genotype I HDV prototype of Italian origin; (2) both forms of the genotype II T3 delta antigen are expressed; and (3) T3 HDV undergoes RNA editing during replication, with 4.8% of the T3 genomes showing evidence of editing. The low level of RNA replication may be related to the milder clinical outcomes of genotype II HDV infections.

Original languageEnglish
Pages (from-to)966-972
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number4
DOIs
StatePublished - 11 07 2003

Keywords

  • Delta antigen
  • HDV genotype II
  • RNA editing

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