TY - JOUR
T1 - Intense pulsed light effects on the expression of extracellular matrix proteins and transforming growth factor beta-1 in skin dermal fibroblasts cultured within contracted collagen lattices
AU - Wong, Wen Rou
AU - Shyu, Wen Ling
AU - Tsai, Jung Wei
AU - Hsu, Kuang Hung
AU - Pang, Jong Hwei S.
PY - 2009/5
Y1 - 2009/5
N2 - BACKGROUND Emerging clinical evidence suggests that intense pulsed light (IPL) treatment may exert some beneficial effects on photoaged skin. The molecular mechanisms underlying this IPL effect have not been fully elucidated. OBJECTIVE To examine the effects of IPL irradiation on normal human dermal fibroblasts grown in contracted collagen lattices. METHODS Human skin fibroblasts cultured in contracted collagen lattices were irradiated with IPL with triple pulses of 7 ms with a pulse interval of 70 ms and fluences of 20, 50, and 75 J/cm2. Twenty-four hours after the irradiation, cell viability, messenger RNA (mRNA), and protein levels of extracellular matrix proteins (e.g., collagen I, collagen III, and fibronectin) and transforming growth factor beta-1 (TGF-β1) were evaluated using dye exclusion, real-time reverse transcriptase polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively. RESULTS A dose-dependent increase in viable cells was demonstrated after the IPL irradiation. There was no significant change in mRNA levels of collagen I and fibronectin. Upregulated expression of collagen III and TGF-β1 in dermal fibroblasts was verified. CONCLUSIONS The analytical results presented here provide a potential mechanistic explanation for the mechanism of clinical photorejuvenation effects of IPL that involves the increase of extracellular matrix construction by upregulating the gene expressions of collagen III and TGF-β1.
AB - BACKGROUND Emerging clinical evidence suggests that intense pulsed light (IPL) treatment may exert some beneficial effects on photoaged skin. The molecular mechanisms underlying this IPL effect have not been fully elucidated. OBJECTIVE To examine the effects of IPL irradiation on normal human dermal fibroblasts grown in contracted collagen lattices. METHODS Human skin fibroblasts cultured in contracted collagen lattices were irradiated with IPL with triple pulses of 7 ms with a pulse interval of 70 ms and fluences of 20, 50, and 75 J/cm2. Twenty-four hours after the irradiation, cell viability, messenger RNA (mRNA), and protein levels of extracellular matrix proteins (e.g., collagen I, collagen III, and fibronectin) and transforming growth factor beta-1 (TGF-β1) were evaluated using dye exclusion, real-time reverse transcriptase polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively. RESULTS A dose-dependent increase in viable cells was demonstrated after the IPL irradiation. There was no significant change in mRNA levels of collagen I and fibronectin. Upregulated expression of collagen III and TGF-β1 in dermal fibroblasts was verified. CONCLUSIONS The analytical results presented here provide a potential mechanistic explanation for the mechanism of clinical photorejuvenation effects of IPL that involves the increase of extracellular matrix construction by upregulating the gene expressions of collagen III and TGF-β1.
UR - http://www.scopus.com/inward/record.url?scp=65349163534&partnerID=8YFLogxK
U2 - 10.1111/j.1524-4725.2009.01138.x
DO - 10.1111/j.1524-4725.2009.01138.x
M3 - 文章
C2 - 19389097
AN - SCOPUS:65349163534
SN - 1076-0512
VL - 35
SP - 816
EP - 825
JO - Dermatologic Surgery
JF - Dermatologic Surgery
IS - 5
ER -