Interaction between NBS1 and the mTOR/Rictor/SIN1 Complex through Specific Domains

Jian Qiu Wang; Jian Hong Chen; Yen Chung Chen; Mei Yu Chen; Chia Ying Hsieh; Shu Chun Teng; Kou Juey Wu

doi:10.1371/journal.pone.0065586

Interaction between NBS1 and the mTOR/Rictor/SIN1 Complex through Specific Domains

Jian Qiu Wang
, Jian Hong Chen
, Yen Chung Chen
, Mei Yu Chen
, Chia Ying Hsieh
, Shu Chun Teng
, Kou Juey Wu^*

^*Corresponding author for this work

National Yang Ming Chiao Tung University
Hangzhou Normal University
Taiwan Advance Biopharm, Inc.
National Taiwan University

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity.

Original language	English
Article number	e65586
Journal	PLoS ONE
Volume	8
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0065586
State	Published - 06 06 2013
Externally published	Yes

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SDG 3 Good Health and Well-being

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@article{6ab2ba2b85bc47788a98da70593fb242,

title = "Interaction between NBS1 and the mTOR/Rictor/SIN1 Complex through Specific Domains",

abstract = "Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity.",

author = "Wang, \{Jian Qiu\} and Chen, \{Jian Hong\} and Chen, \{Yen Chung\} and Chen, \{Mei Yu\} and Hsieh, \{Chia Ying\} and Teng, \{Shu Chun\} and Wu, \{Kou Juey\}",

year = "2013",

month = jun,

day = "6",

doi = "10.1371/journal.pone.0065586",

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T1 - Interaction between NBS1 and the mTOR/Rictor/SIN1 Complex through Specific Domains

AU - Wang, Jian Qiu

AU - Chen, Jian Hong

AU - Chen, Yen Chung

AU - Chen, Mei Yu

AU - Hsieh, Chia Ying

AU - Teng, Shu Chun

AU - Wu, Kou Juey

PY - 2013/6/6

Y1 - 2013/6/6

N2 - Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity.

AB - Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity.

UR - https://www.scopus.com/pages/publications/84878781864

U2 - 10.1371/journal.pone.0065586

DO - 10.1371/journal.pone.0065586

M3 - 文章

C2 - 23762398

AN - SCOPUS:84878781864

SN - 1932-6203

VL - 8

JO - PLoS ONE

JF - PLoS ONE

IS - 6

M1 - e65586

ER -

Interaction between NBS1 and the mTOR/Rictor/SIN1 Complex through Specific Domains

Abstract

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