Interaction of human thymidine kinase 1 with p21Waf1

D. Y. Huan, Z. F. Chang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

10 Scopus citations

Abstract

The overexpression of the cyclin-dependent kinase (CDK) inhibitor p21Waf1 can inhibit cell proliferation, which is mediated by direct binding to CDK and proliferating-cell nuclear antigen. In this study, we demonstrated that human cytosolic thymidine kinase 1 (TK1) polypeptide can form a complex with p21Waf1. The C-terminal domain of p21Waf1 appeared to interact with the TK1 polypeptide, but, despite the inhibitory function of p21Waf1, their association did not alter TK1 functional activity. However, overexpression of TK1 overcame p21Waf1-mediated growth suppression and blocked the association of CDK2 with p21Waf1, suggesting that TK1 interferes with the inhibitory function of p21Waf1. Based on these results, we here propose that the molecular function of p21Waf1 in cells can be perturbed through its interaction with another cellular protein, TK1.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalBiochemical Journal
Volume356
Issue number3
DOIs
StatePublished - 15 06 2001
Externally publishedYes

Keywords

  • Cell cycle
  • Cell growth
  • Protein interaction

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