TY - JOUR
T1 - Intra-coronary administration of cyclosporine limits infarct size, attenuates remodeling and preserves left ventricular function in porcine acute anterior infarction
AU - Sheu, Jiunn Jye
AU - Chua, Sarah
AU - Sun, Cheuk Kwan
AU - Chang, Li Teh
AU - Yen, Chia Hung
AU - Wu, Chiung Jen
AU - Fu, Morgan
AU - Yip, Hon Kan
PY - 2011/2/17
Y1 - 2011/2/17
N2 - Background: We tested whether intra-coronary administration of cyclosporine effectively preserves left ventricular (LV) function in porcine acute anterior wall infarction (AMI). Methods and results: Eighteen male mini-pigs were randomized into groups 1 (control), 2 (AMI alone), and 3 (AMI with cyclosporine treatment). AMI was induced by ligating middle left anterior descending artery (LAD). Fifteen minutes after ligation, saline and cyclosporine (2.5 mg) combination was injected into LAD beyond ligation in groups 2 and 3, respectively. Echocardiography was performed after 14 days, followed by animal sacrifice. Larger infarcted area (IA) was noted in group 2 than in group 3 (p < 0.001). In both IA and peri-IA, mRNA expressions of IL-8, MMP-9, caspase 3 and Bax were higher, whereas PGC-1α, eNOS and Bcl-2 were lower in group 2 than in groups 1 and 3 (p < 0.01). The mRNA expression of IL-10 was upregulated in both IA and peri-IA in group 3 compared with groups 1 and 2. Apoptotic nuclei and CD40-positive cells were higher in both peri-IA and non-IA in group 2 than in groups 1 and 3 (p < 0.001). Oxidative stress and cytosolic cytochrome C in IA were increased in group 2 than in groups 1 and 3 (p < 0.001). Mid-LV end-systolic areas were higher, whereas mid-LV wall fractional area change was lower in group 2 than in groups 1 and 3 (p < 0.001). Conclusions: Intra-coronary administration of cyclosporine effectively limits infarct size, attenuates LV remodeling and preserves LV function.
AB - Background: We tested whether intra-coronary administration of cyclosporine effectively preserves left ventricular (LV) function in porcine acute anterior wall infarction (AMI). Methods and results: Eighteen male mini-pigs were randomized into groups 1 (control), 2 (AMI alone), and 3 (AMI with cyclosporine treatment). AMI was induced by ligating middle left anterior descending artery (LAD). Fifteen minutes after ligation, saline and cyclosporine (2.5 mg) combination was injected into LAD beyond ligation in groups 2 and 3, respectively. Echocardiography was performed after 14 days, followed by animal sacrifice. Larger infarcted area (IA) was noted in group 2 than in group 3 (p < 0.001). In both IA and peri-IA, mRNA expressions of IL-8, MMP-9, caspase 3 and Bax were higher, whereas PGC-1α, eNOS and Bcl-2 were lower in group 2 than in groups 1 and 3 (p < 0.01). The mRNA expression of IL-10 was upregulated in both IA and peri-IA in group 3 compared with groups 1 and 2. Apoptotic nuclei and CD40-positive cells were higher in both peri-IA and non-IA in group 2 than in groups 1 and 3 (p < 0.001). Oxidative stress and cytosolic cytochrome C in IA were increased in group 2 than in groups 1 and 3 (p < 0.001). Mid-LV end-systolic areas were higher, whereas mid-LV wall fractional area change was lower in group 2 than in groups 1 and 3 (p < 0.001). Conclusions: Intra-coronary administration of cyclosporine effectively limits infarct size, attenuates LV remodeling and preserves LV function.
KW - Acute myocardial infarction
KW - Intra-coronary administration of cyclosporine
KW - Mini-pig model
UR - http://www.scopus.com/inward/record.url?scp=79951727149&partnerID=8YFLogxK
U2 - 10.1016/j.ijcard.2009.08.008
DO - 10.1016/j.ijcard.2009.08.008
M3 - 文章
C2 - 19751953
AN - SCOPUS:79951727149
SN - 0167-5273
VL - 147
SP - 79
EP - 87
JO - International Journal of Cardiology
JF - International Journal of Cardiology
IS - 1
ER -