Intrathecal administration of roscovitine inhibits Cdk5 activity and attenuates formalin-induced nociceptive in rats

Cheng Haung Wang; Wen Ying Chou; Kung Sheng Hung; Bruno Jawan; Cheng Nann Lu; Jong Kang Liu; Yi Ping Hung; Tsung Hsing Lee

doi:10.1111/j.1745-7254.2005.00007.x

Intrathecal administration of roscovitine inhibits Cdk5 activity and attenuates formalin-induced nociceptive in rats

Cheng Haung Wang, Wen Ying Chou, Kung Sheng Hung, Bruno Jawan, Cheng Nann Lu, Jong Kang Liu, Yi Ping Hung, Tsung Hsing Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

32 Scopus citations

Abstract

Aim: To investigate effects of the cyclin-dependent kinase5 (Cdk5) inhibitor roscovitine on formalin-induced nociceptive responses in rats. Methods: The flinch response as a methood of pain threshold measurement and intrathecal injection techniques were used. Cdk5 and phosphorylation of its downstream target, DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of M_r 32 kDa), were investigated by Western blot analysis. Results: Rats demonstrated a typical flinch response after formalin injection. Intrathecal roscovitine injections significantly suppressed the flinch response in a dose-dependent manner. Western blot analysis showed that phosphorylated DARPP-32 at Thr75 increased in concentration after formalin hyperalgesia, with this effect reduced by roscovitine administration. This antinociception was partially attenuated by administration of naloxone before the formalin test. Conclusion: DARPP-32 phosphorylation is involved in acute inflammatory pain response. Intrathecal roscovitine administration attenuates formalin-induced nociceptive responses and there is potential for further application.

Original language	English
Pages (from-to)	46-50
Number of pages	5
Journal	Acta Pharmacologica Sinica
Volume	26
Issue number	1
DOIs	https://doi.org/10.1111/j.1745-7254.2005.00007.x
State	Published - 01 2005
Externally published	Yes

Keywords

Cyclin-dependent kinase-5
DARPP-32
Roscovitine

Access to Document

10.1111/j.1745-7254.2005.00007.x

Cite this

@article{46cc7c4042d64cfc9177e9b752cc101d,

title = "Intrathecal administration of roscovitine inhibits Cdk5 activity and attenuates formalin-induced nociceptive in rats",

abstract = "Aim: To investigate effects of the cyclin-dependent kinase5 (Cdk5) inhibitor roscovitine on formalin-induced nociceptive responses in rats. Methods: The flinch response as a methood of pain threshold measurement and intrathecal injection techniques were used. Cdk5 and phosphorylation of its downstream target, DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of Mr 32 kDa), were investigated by Western blot analysis. Results: Rats demonstrated a typical flinch response after formalin injection. Intrathecal roscovitine injections significantly suppressed the flinch response in a dose-dependent manner. Western blot analysis showed that phosphorylated DARPP-32 at Thr75 increased in concentration after formalin hyperalgesia, with this effect reduced by roscovitine administration. This antinociception was partially attenuated by administration of naloxone before the formalin test. Conclusion: DARPP-32 phosphorylation is involved in acute inflammatory pain response. Intrathecal roscovitine administration attenuates formalin-induced nociceptive responses and there is potential for further application.",

keywords = "Cyclin-dependent kinase-5, DARPP-32, Roscovitine",

author = "Wang, {Cheng Haung} and Chou, {Wen Ying} and Hung, {Kung Sheng} and Bruno Jawan and Lu, {Cheng Nann} and Liu, {Jong Kang} and Hung, {Yi Ping} and Lee, {Tsung Hsing}",

year = "2005",

month = jan,

doi = "10.1111/j.1745-7254.2005.00007.x",

language = "英语",

volume = "26",

pages = "46--50",

journal = "Acta Pharmacologica Sinica",

issn = "1671-4083",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - Intrathecal administration of roscovitine inhibits Cdk5 activity and attenuates formalin-induced nociceptive in rats

AU - Wang, Cheng Haung

AU - Chou, Wen Ying

AU - Hung, Kung Sheng

AU - Jawan, Bruno

AU - Lu, Cheng Nann

AU - Liu, Jong Kang

AU - Hung, Yi Ping

AU - Lee, Tsung Hsing

PY - 2005/1

Y1 - 2005/1

N2 - Aim: To investigate effects of the cyclin-dependent kinase5 (Cdk5) inhibitor roscovitine on formalin-induced nociceptive responses in rats. Methods: The flinch response as a methood of pain threshold measurement and intrathecal injection techniques were used. Cdk5 and phosphorylation of its downstream target, DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of Mr 32 kDa), were investigated by Western blot analysis. Results: Rats demonstrated a typical flinch response after formalin injection. Intrathecal roscovitine injections significantly suppressed the flinch response in a dose-dependent manner. Western blot analysis showed that phosphorylated DARPP-32 at Thr75 increased in concentration after formalin hyperalgesia, with this effect reduced by roscovitine administration. This antinociception was partially attenuated by administration of naloxone before the formalin test. Conclusion: DARPP-32 phosphorylation is involved in acute inflammatory pain response. Intrathecal roscovitine administration attenuates formalin-induced nociceptive responses and there is potential for further application.

AB - Aim: To investigate effects of the cyclin-dependent kinase5 (Cdk5) inhibitor roscovitine on formalin-induced nociceptive responses in rats. Methods: The flinch response as a methood of pain threshold measurement and intrathecal injection techniques were used. Cdk5 and phosphorylation of its downstream target, DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of Mr 32 kDa), were investigated by Western blot analysis. Results: Rats demonstrated a typical flinch response after formalin injection. Intrathecal roscovitine injections significantly suppressed the flinch response in a dose-dependent manner. Western blot analysis showed that phosphorylated DARPP-32 at Thr75 increased in concentration after formalin hyperalgesia, with this effect reduced by roscovitine administration. This antinociception was partially attenuated by administration of naloxone before the formalin test. Conclusion: DARPP-32 phosphorylation is involved in acute inflammatory pain response. Intrathecal roscovitine administration attenuates formalin-induced nociceptive responses and there is potential for further application.

KW - Cyclin-dependent kinase-5

KW - DARPP-32

KW - Roscovitine

UR - http://www.scopus.com/inward/record.url?scp=11844303490&partnerID=8YFLogxK

U2 - 10.1111/j.1745-7254.2005.00007.x

DO - 10.1111/j.1745-7254.2005.00007.x

M3 - 文章

C2 - 15659113

AN - SCOPUS:11844303490

SN - 1671-4083

VL - 26

SP - 46

EP - 50

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

IS - 1

ER -

Intrathecal administration of roscovitine inhibits Cdk5 activity and attenuates formalin-induced nociceptive in rats

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this