Intrathecal miR-183 delivery suppresses mechanical allodynia in mononeuropathic rats

Chung Ren Lin*, Kuan Hung Chen, Chien Hui Yang, Hui Wen Huang, Shyr Ming Sheen-Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

50 Scopus citations


Members of the miR-183 family are unique in that they are highly abundant in sensory organs. In a recent study, significant downregulation was observed for miR-96 and miR-183 in the L5 dorsal root ganglion (DRG) 2 weeks after spinal nerve ligation (SNL). In this study, we focused on miR-183, which is the most regulated member of the miR-183 family, to look at the specific role on neuropathic pain. Persistent mechanical allodynia was induced with the L5 SNL model in 8-week-old male Sprague-Dawley rats. Paw withdrawal thresholds in response to mechanical stimuli were assessed with Von Frey filaments. Expression of miR-183 in the L5 DRG was assessed with quantitative real-time polymerase chain reaction (qPCR) analysis. Lentivirions expressing miR-183 were injected intrathecally into SNL rats. Changes in mechanical allodynia were assessed with Von Frey filaments. In addition, changes in the predicted target genes of miR-183 were assessed with qPCR. L5 SNL produced marked mechanical allodynia in the ipsilateral hindpaws of adult rats, beginning at postoperative day 1 and continuing to day 14. L5 SNL caused significant downregulation of miR-183 in adult DRG cells. Intrathecal administration of lentivirions expressing miR-183 downregulated SNL-induced increases in the expression of Nav1.3 and brain-derived neurotrophic factor (BDNF), which correlated with the significant attenuation of SNL-induced mechanical allodynia.

Original languageEnglish
Pages (from-to)1682-1689
Number of pages8
JournalEuropean Journal of Neuroscience
Issue number10
StatePublished - 05 2014


  • Intrathecal delivery
  • Lentivirions
  • Neuropathic pain
  • Spinal nerve ligation


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