Investigation of effect of tectorigenin (O-methylated isoflavone) on Ca²⁺ signal transduction and cytotoxic responses in canine renal tubular cells

Tectorigenin, a traditional Chinese medicine, is isolated from the flower of plants such as Pueraria thomsonii Benth. It is an O-methylated isoflavone, a type of flavonoid. Previous studies have shown that tectorigenin evoked various physiological responses in different models, but the effect of tectorigenin on cytosolic-free Ca²⁺ levels ([Ca²⁺]_i) and cytotoxicity in renal tubular cells is unknown. Our research explored if tectorigenin changed Ca²⁺ signal transduction and viability in Madin-Darby Canine Kidney (MDCK) renal tubular cells. [Ca²⁺]_i in suspended cells were measured by applying the fluorescent Ca²⁺-sensitive probe fura-2. Viability was explored by using water-soluble tetrazolium-1 as a fluorescent dye. Tectorigenin at concentrations of 5-50 μM induced [Ca²⁺]_i rises. Ca²⁺ removal reduced the signal by approximately 20%. Tectorigenin (50 μM) induced Mn²⁺ influx suggesting of Ca²⁺ entry. Tectorigenin-induced Ca²⁺ entry was inhibited by 10% by three inhibitors of store-operated Ca²⁺ channels, namely, nifedipine, econazole, and SKF96365. In Ca²⁺-free medium, treatment with the endoplasmic reticulum Ca²⁺ pump inhibitor thapsigargin inhibited 83% of tectorigenin-evoked [Ca²⁺]_i rises. Conversely, treatment with tectorigenin abolished thapsigargin-evoked [Ca²⁺]_i rises. Inhibition of phospholipase C with U73122 inhibited 50% of tectorigenin-induced [Ca²⁺]_i rises. Tectorigenin at concentrations between 10 and 60 μM killed cells in a concentration-dependent fashion. Chelation of cytosolic Ca²⁺ with 1,2-bis (2-aminophenoxy)ethane-N, N, N', N'-tetraacetic acid/acetoxy methyl did not reverse tectorigenin's cytotoxicity. Our data suggest that, in MDCK cells, tectorigenin evoked [Ca²⁺]_i rises and induced cell death that was not associated with [Ca²⁺]_i rises. Therefore, tectorigenin may be a Ca²⁺-independent cytotoxic agent for kidney cells.