Investigation of the Effect of Acoustic Pressure and Sonication Duration on Focused-Ultrasound Induced Blood-Brain Barrier Disruption

P. C. Chu, M. C. Hsiao, Y. H. Yang, J. C. Chen, H. L. Liu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

The purpose of this study is to investigate the influence of the ultrasound peak pressure and sonication duration on blood-brain barrier (BBB) disruption, and also seek for the optimized sonication parameter to introduce BBB-opening with minimal hemorrhagic damage. Twelve SD rats were sonicated. During sonication, the 309 kHz focused ultrasound was employed to deliver burst-mode low-pressure ultrasound to animal brains in the presence of microbubbles to introduce local and temporal BBB disruption. Different ultrasonic peak pressures and sonication durations were tested, and the BBB-disruption and accompanied hemorrhage were confirmed by brain section and histological examinations. Our results showed that the peak pressure is the major factor to generate BBB disruption, where as the sonication duration can be utilized to moderately regulate the accompanied hemorrhagic damage. We also found that the low-pressure long-duration ultrasound exposure may be beneficiary to reduce hemorrhage than high-pressure short-duration mode.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages1094-1097
Number of pages4
DOIs
StatePublished - 2009
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: 03 12 200806 12 2008

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
Country/TerritorySingapore
Period03/12/0806/12/08

Keywords

  • Focused ultrasound
  • blood-brain barrier
  • histology

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