TY - JOUR
T1 - Involvement of nucleophosmin/B23 in TPA-induced megakaryocytic differentiation of K562 cells
AU - Hsu, C. Y.
AU - Yung, Bym
PY - 2003/10/6
Y1 - 2003/10/6
N2 - Human myelogenous leukaemia K562 cells were induced to undergo megakaryocytic differentiation by treatment with phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) (20 nM, 24-72 h). The steady-state level of nucleophosmin/B23 mRNA decreased during the TPA-induced differentiation. There was also decrease in the level of cellular nucleophosmin/B23 protein and appearance of its degraded product (25 kDa) during the TPA-induced differentiation. Furthermore, K562/B23 (wild type), K562/D1 (Δ280-294) and K562/D2 (Δ263-294) cells were less, while K562/D3 (Δ244-294) cells were more responsive to TPA-induced differentiation as compared to K562/vector or parental K562 cells. Activation of the ERK/MAPK was observed in parental K562 cells upon TPA treatment (5 nM, 5-30 min). As compared to K562/vector cells, less activation of ERK/MAPK was observed in K562/D2 cells, while ERK/MAPK was highly activated in K562/D3 cells upon TPA treatment. Our results indicate that nucleophosmin/B23 plays an important role in TPA-induced differentiation of K562 cells and the amino acids 244-294 at C-terminal of nucleophosmin/B23 could be an important site for regulation of cellular response to differentiation.
AB - Human myelogenous leukaemia K562 cells were induced to undergo megakaryocytic differentiation by treatment with phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) (20 nM, 24-72 h). The steady-state level of nucleophosmin/B23 mRNA decreased during the TPA-induced differentiation. There was also decrease in the level of cellular nucleophosmin/B23 protein and appearance of its degraded product (25 kDa) during the TPA-induced differentiation. Furthermore, K562/B23 (wild type), K562/D1 (Δ280-294) and K562/D2 (Δ263-294) cells were less, while K562/D3 (Δ244-294) cells were more responsive to TPA-induced differentiation as compared to K562/vector or parental K562 cells. Activation of the ERK/MAPK was observed in parental K562 cells upon TPA treatment (5 nM, 5-30 min). As compared to K562/vector cells, less activation of ERK/MAPK was observed in K562/D2 cells, while ERK/MAPK was highly activated in K562/D3 cells upon TPA treatment. Our results indicate that nucleophosmin/B23 plays an important role in TPA-induced differentiation of K562 cells and the amino acids 244-294 at C-terminal of nucleophosmin/B23 could be an important site for regulation of cellular response to differentiation.
KW - Megakaryocytic differentiation
KW - Nucleophosmin/B23
KW - TPA
UR - http://www.scopus.com/inward/record.url?scp=0242329766&partnerID=8YFLogxK
U2 - 10.1038/sj.bjc.6601100
DO - 10.1038/sj.bjc.6601100
M3 - 文章
C2 - 14520467
AN - SCOPUS:0242329766
SN - 0007-0920
VL - 89
SP - 1320
EP - 1326
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 7
ER -
