Isolation and characterization of plasmid pSW200 from Erwinia stewartii

Jen Fen Fu; Jer Ming Hu; Yu Sun Chang; Shih Tung Liu

doi:10.1006/plas.1998.1350

Isolation and characterization of plasmid pSW200 from Erwinia stewartii

Jen Fen Fu
, Jer Ming Hu
, Yu Sun Chang
, Shih Tung Liu^*

^*Corresponding author for this work

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

The nucleotide sequence of pSW200 of Erwinia stewartii SW2 was determined. This plasmid is 4367 bp long, consisting of four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1- type plasmids. The plasmid also contains a region of forty-one 15-bp repeats. Deleting this region does not affect the stability or the copy number when maintained as sole plasmid in the cell. However, the plasmid is rapidly lost when a homoplasmid with the intact repeat region is introduced into the cell. The function of this region may provide pSW200 an advantage in competing with an incompatible plasmid in the cell.

Original language	English
Pages (from-to)	100-112
Number of pages	13
Journal	Plasmid
Volume	40
Issue number	2
DOIs	https://doi.org/10.1006/plas.1998.1350
State	Published - 09 1998

Keywords

ColE1-type plasmid
Erwinia stewartii
Mobilization
Plasmid competition

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10.1006/plas.1998.1350

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title = "Isolation and characterization of plasmid pSW200 from Erwinia stewartii",

abstract = "The nucleotide sequence of pSW200 of Erwinia stewartii SW2 was determined. This plasmid is 4367 bp long, consisting of four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1- type plasmids. The plasmid also contains a region of forty-one 15-bp repeats. Deleting this region does not affect the stability or the copy number when maintained as sole plasmid in the cell. However, the plasmid is rapidly lost when a homoplasmid with the intact repeat region is introduced into the cell. The function of this region may provide pSW200 an advantage in competing with an incompatible plasmid in the cell.",

keywords = "ColE1-type plasmid, Erwinia stewartii, Mobilization, Plasmid competition",

author = "Fu, \{Jen Fen\} and Hu, \{Jer Ming\} and Chang, \{Yu Sun\} and Liu, \{Shih Tung\}",

year = "1998",

month = sep,

doi = "10.1006/plas.1998.1350",

language = "英语",

volume = "40",

pages = "100--112",

journal = "Plasmid",

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T1 - Isolation and characterization of plasmid pSW200 from Erwinia stewartii

AU - Fu, Jen Fen

AU - Hu, Jer Ming

AU - Chang, Yu Sun

AU - Liu, Shih Tung

PY - 1998/9

Y1 - 1998/9

N2 - The nucleotide sequence of pSW200 of Erwinia stewartii SW2 was determined. This plasmid is 4367 bp long, consisting of four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1- type plasmids. The plasmid also contains a region of forty-one 15-bp repeats. Deleting this region does not affect the stability or the copy number when maintained as sole plasmid in the cell. However, the plasmid is rapidly lost when a homoplasmid with the intact repeat region is introduced into the cell. The function of this region may provide pSW200 an advantage in competing with an incompatible plasmid in the cell.

AB - The nucleotide sequence of pSW200 of Erwinia stewartii SW2 was determined. This plasmid is 4367 bp long, consisting of four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1- type plasmids. The plasmid also contains a region of forty-one 15-bp repeats. Deleting this region does not affect the stability or the copy number when maintained as sole plasmid in the cell. However, the plasmid is rapidly lost when a homoplasmid with the intact repeat region is introduced into the cell. The function of this region may provide pSW200 an advantage in competing with an incompatible plasmid in the cell.

KW - ColE1-type plasmid

KW - Erwinia stewartii

KW - Mobilization

KW - Plasmid competition

UR - https://www.scopus.com/pages/publications/0031659777

U2 - 10.1006/plas.1998.1350

DO - 10.1006/plas.1998.1350

M3 - 文章

C2 - 9735312

AN - SCOPUS:0031659777

SN - 0147-619X

VL - 40

SP - 100

EP - 112

JO - Plasmid

JF - Plasmid

IS - 2

ER -

Isolation and characterization of plasmid pSW200 from Erwinia stewartii

Abstract

Keywords

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