Isolation and culture of human macrophages.

John Q. Davies*, Siamon Gordon

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

80 Scopus citations


Methods to isolate and culture human monocyte-derived macrophages and alveolar macrophages are described. Monocytes are obtained from buffy-coat preparations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be purified from bronchoalveolar fluid samples by adhesion to tissue culture plastic. If resected lung tissue is available, alveolar macrophages can be obtained by mechanically disrupting the lung parenchyma, followed by adhesion-mediated purification.

Original languageEnglish
Pages (from-to)105-116
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2005
Externally publishedYes


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