Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin

Hsing I. Huang; Chung Zu Wu

doi:10.1007/978-1-61779-815-3_27

Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin

Hsing I. Huang^*
, Chung Zu Wu

^*Corresponding author for this work

Department of Medical Biotechnology and Laboratory Science

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.

Original language	English
Pages (from-to)	465-470
Number of pages	6
Journal	Methods in Molecular Biology
Volume	879
DOIs	https://doi.org/10.1007/978-1-61779-815-3_27
State	Published - 2012

Keywords

Differentiation
Foreskin
Isolation
Stromal cells

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10.1007/978-1-61779-815-3_27

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@article{f0395cde1d534e5aa088d585bbe207ad,

title = "Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin",

abstract = "Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.",

keywords = "Differentiation, Foreskin, Isolation, Stromal cells",

author = "Huang, \{Hsing I.\} and Wu, \{Chung Zu\}",

year = "2012",

doi = "10.1007/978-1-61779-815-3\_27",

language = "英语",

volume = "879",

pages = "465--470",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin

AU - Huang, Hsing I.

AU - Wu, Chung Zu

PY - 2012

Y1 - 2012

N2 - Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.

AB - Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.

KW - Differentiation

KW - Foreskin

KW - Isolation

KW - Stromal cells

UR - https://www.scopus.com/pages/publications/84861805429

U2 - 10.1007/978-1-61779-815-3_27

DO - 10.1007/978-1-61779-815-3_27

M3 - 文章

C2 - 22610576

AN - SCOPUS:84861805429

SN - 1064-3745

VL - 879

SP - 465

EP - 470

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin

Abstract

Keywords

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