Kinetic analysis on membrane-based reverse micellar extraction of lysozyme from aqueous solutions

Ruey Shin Juang*, Hsiang Chien Kao, Chiau Lin Shiau

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

8 Scopus citations

Abstract

The extraction of lysozyme from aqueous solutions through a flat-sheet microporous membrane (pore size 0.45 μm, thickness 147 μm, porosity 0.75) into an isooctane solution of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelles was examined. Batch liquid-liquid extraction experiments were first conducted at different lysozyme concentrations (250-1000 mg L-1), KCl concentrations (0.1-1.2 M), pH (2-12) and AOT concentrations (0.01-0.1 M) to obtain equilibrium relationships. Effective extraction of lysozyme was achieved in the KCl concentration range of 0.1-0.4 M and pH range of 4-9. More than 90% of lysozyme could be stripped to an aqueous phase of high alkalinity (pH 11.5) and high KCl concentration (1.5 M). A mass transfer model was proposed that considers all diffusion in the aqueous stagnant layer, membrane and reverse micellar stagnant layer to predict the transport flux of lysozyme in the present membrane-based extraction process. The solubilization of lysozyme from aqueous phase to the AOT/isooctane reverse micelles was assumed to attain equilibrium instantaneously. A good agreement between the calculated and measured fluxes was obtained under the ranges studied (standard deviation, 11%).

Original languageEnglish
Pages (from-to)636-645
Number of pages10
JournalJournal of Membrane Science
Volume281
Issue number1-2
DOIs
StatePublished - 15 09 2006
Externally publishedYes

Keywords

  • AOT
  • Lysozyme
  • Mass transfer modeling
  • Membrane-based extraction process
  • Reverse micellar extraction

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