Abstract
A dual-frequency equal-amplitude paired polarization heterodyne Polarimeter (DEPHP) was set up in order to precisely measure the mutarotation rate constants of D-glucose in tridistilled water. The DEPHP is based on a balanced detector detection scheme for measurement of the optical rotation angle of D-glucose/water solution during the conversion process between α-D-glucose and β-D-glucose while in a nonequilibrium state. The DEPHP can perform shot-noise-limited detection, so that the total, optical rotation angle together with the mutarotation rate constants of α-D-glucose and β-D-glucose conversion can be measured with high sensitivity. In this experiment, the sensitivity of the optical rotation angle measurement was 8.3 × 10 -5 deg/ cm, while the total (k), forward (k1 and reverse (k2) mutarotation rate constants of D-glucose were found to be k = 7.67 × 10-5 s-1 k1 = 2.76 × 10 -5 s-1, and k2 = 4.91 × 10-5 s-1 respectively, in tridistilled water. Moreover, using the DEPHP, we can measure the specific rotation angles of α-D-glucose and β-D-glucose in water at 632.8 nm. They were 105° and 12°, respectively. Finally, the detection sensitivity of the DEPHP system is also discussed.
Original language | English |
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Pages (from-to) | 1665-1669 |
Number of pages | 5 |
Journal | Journal of Physical Chemistry A |
Volume | 114 |
Issue number | 4 |
DOIs | |
State | Published - 2010 |