Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

Jim Long Her, Tung Ming Pan*, Wan Ying Lin, Kai Sheng Wang, Lain Jong Li

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

30 Scopus citations


In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R2 = 0.99) in the ALT concentration range of 10-100 U/L.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalSensors and Actuators, B: Chemical
StatePublished - 2013


  • Alanine aminotransferase (ALT)
  • Graphene field-effect transistor (GFET)
  • l-Alanine
  • α-Ketoglutarate


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