Label-free detection of DNA using high-κ Lu 2Ti 2O 7 electrolyte-insulator-semiconductors

Tung Ming Pan*, Kung Yuan Chang, Chao Wen Lin, Shiao Wen Tsai, Min Hsien Wu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

14 Scopus citations


In this study, we propose a disposable poly-N-isopropylacrylamide (PNIPAAm) as an immobilizing biological material based electrolyte-insulator- semiconductor (EIS) device for DNA detection, prepared from a high-κ Lu 2Ti 2O 7 sensing membrane. X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy were used to investigate the structural and morphological features of these films annealed at various annealing temperatures. The high-κ Lu 2Ti 2O 7 EIS device annealed at 900 °C exhibited a higher sensitivity of 60.04 mV/pH, a smaller hysteresis voltage of 3 mV, and a lower drift rate of 0.623 mV h -1 than those at other annealing temperatures. These results are attributed to the large surface roughness and the small number of crystal defects. In the biosensing experiments, the immobilization of a negatively charged single-stranded DNA probe (ssDNA) on the Lu 2Ti 2O 7 surface caused an increase in the reference voltage value, whereas the hybridization of complementary target DNA (cDNA) decreased the reference voltage value. The Lu 2Ti 2O 7 EIS biosensor using a ssDNA-immobilized PNIPAAm gel was successfully used for the detection of DNA hybridization. The detection limit of complementary DNA was as low as 10 pM, and the output signal of the Lu 2Ti 2O 7 biosensor increased linearly with the logarithm of cDNA concentration in the range of 10 -11 to 10 -4 M.

Original languageEnglish
Pages (from-to)1358-1363
Number of pages6
JournalJournal of Materials Chemistry
Issue number4
StatePublished - 28 01 2012


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