TY - JOUR
T1 - Label-free detection of uric acid using a disposable poly-N- isopropylacrylamide as an encapsulating enzyme material based on high-κ Eu2Ti2O7 electrolyte-insulator-semiconductor devices
AU - Pan, Tung Ming
AU - Chang, Kung Yuan
AU - Lin, Chao Wen
AU - Tsai, Shiao Wen
AU - Wu, Min Hsien
PY - 2011/12/15
Y1 - 2011/12/15
N2 - In this paper, we proposed a disposable poly-N-isopropylacrylamide (PNIPAAm) as an encapsulating enzyme material based an electrolyte-insulator- semiconductor (EIS) device for uric acid biosensing, prepared from high-κ Eu2Ti2O7 sensing membranes. We used X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy to examine the structural and morphological features of these membranes annealed at different annealing temperatures. The Eu2Ti2O7 EIS device annealed at 900 °C exhibited a higher sensitivity (59.03 mV/pH), a lower drift rate (0.586 mV/h), and a smaller hysteresis voltage (2.8 mV) compared to those of the other annealing temperatures, presumably because of its lower number of crystal defects and its higher surface roughness. Furthermore, for a high-κ Eu2Ti2O7 EIS biosensor, a disposable PNIPAAm was used as an encapsulating enzyme material, making the loading and removal of a biological recognition element on a biosensor easily. PNIPAAm film has reversible solubility to become a solution-gelatin state change in response to thermal condition (∼32 °C). Below the phase transition temperature, such a PNIPAAm film exhibits a higher solubility in water, whereas this film becomes a gel state if the temperature increases. The sensitivity of an uricase-immobilized EIS biosensor was 17.6 mV/mM for uric acid concentrations ranging from 2 to 8 mM.
AB - In this paper, we proposed a disposable poly-N-isopropylacrylamide (PNIPAAm) as an encapsulating enzyme material based an electrolyte-insulator- semiconductor (EIS) device for uric acid biosensing, prepared from high-κ Eu2Ti2O7 sensing membranes. We used X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy to examine the structural and morphological features of these membranes annealed at different annealing temperatures. The Eu2Ti2O7 EIS device annealed at 900 °C exhibited a higher sensitivity (59.03 mV/pH), a lower drift rate (0.586 mV/h), and a smaller hysteresis voltage (2.8 mV) compared to those of the other annealing temperatures, presumably because of its lower number of crystal defects and its higher surface roughness. Furthermore, for a high-κ Eu2Ti2O7 EIS biosensor, a disposable PNIPAAm was used as an encapsulating enzyme material, making the loading and removal of a biological recognition element on a biosensor easily. PNIPAAm film has reversible solubility to become a solution-gelatin state change in response to thermal condition (∼32 °C). Below the phase transition temperature, such a PNIPAAm film exhibits a higher solubility in water, whereas this film becomes a gel state if the temperature increases. The sensitivity of an uricase-immobilized EIS biosensor was 17.6 mV/mM for uric acid concentrations ranging from 2 to 8 mM.
KW - Electrolyte-insulator-semiconductor (EIS)
KW - EuTi O
KW - Poly-N-isopropylacrylamide (PNIPAAm)
KW - Uric acid
KW - pH sensitivity
UR - http://www.scopus.com/inward/record.url?scp=81155148225&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2011.08.071
DO - 10.1016/j.snb.2011.08.071
M3 - 文章
AN - SCOPUS:81155148225
SN - 0925-4005
VL - 160
SP - 850
EP - 857
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1
ER -