TY - JOUR
T1 - Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis
AU - Lin, Yang Wei
AU - Chiu, Tai Chia
AU - Chang, Huan Tsung
PY - 2003/8/5
Y1 - 2003/8/5
N2 - Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.
AB - Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.
KW - DNA
KW - Proteins
KW - Reviews
UR - http://www.scopus.com/inward/record.url?scp=0038106470&partnerID=8YFLogxK
U2 - 10.1016/S1570-0232(03)00363-5
DO - 10.1016/S1570-0232(03)00363-5
M3 - 文献综述
C2 - 12880853
AN - SCOPUS:0038106470
SN - 1570-0232
VL - 793
SP - 37
EP - 48
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -