Laser sintered porous polycaprolacone scaffolds loaded with hyaluronic acid and gelatin-grafted thermoresponsive hydrogel for cartilage tissue engineering

Ming-Yih Lee*, Wen Wei Tsai, His Jung Chen, Jyh Ping Chen, Chih Hao Chen, Wen Lin Yeh, Jia An

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

21 Scopus citations

Abstract

The aim of this study is to evaluate a soft/hard bi-phase scaffold for cartilage tissue engineering. Chondrocyte proliferation, glycoaminoglycan production and total collagen content are compared between laser-sintered porous polycaprolactone (PCL) scaffolds with and without a thermoresponsive hydrogel grafted with hyaluronic acid and gelatin. The in vitro results show that scaffolds loaded with hydrogel have a higher initial chondrocyte attachment than PCL scaffolds. At day 21 and 28, scaffolds loaded with hydrogel have a significantly higher glycosaminoglycan (GAG) production than PCL scaffolds alone, and total collagen content including collagen type II in the hydrogel-loaded group is three times higher than the group without hydrogel. It is concluded that the laser-sintered porous PCL scaffold has good cytocompatibility, and that the hydrogel phase is able to enhance initial chondrocytes attachment as well as GAG and collagen production of chondrocytes. This study suggests that a soft/hard bi-phase scaffold may be used for cartilage tissue engineering to enhance in vitro chondrogenesis.

Original languageEnglish
Pages (from-to)533-543
Number of pages11
JournalBio-Medical Materials and Engineering
Volume23
Issue number6
DOIs
StatePublished - 2013

Keywords

  • Rapid prototyping
  • cartilage tissue engineering
  • chondrocyte
  • gelatin
  • hyaluronic acid
  • selective laser sintering
  • thermoresponsive hydrogel

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