Lectin and anti-carbohydrate antibody assays using chemically modified ligands

Maria Duk; Albert M. Wu; Elwira Lisowska

doi:10.1007/978-1-4615-1267-7_9

Lectin and anti-carbohydrate antibody assays using chemically modified ligands

Maria Duk^*
, Albert M. Wu
, Elwira Lisowska

^*Corresponding author for this work

Department of Molecular Biology

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Microtiter plate assays and 'lectinoblotting' with the use of biotinylated lectins are sensitive and easy to perform methods that can be combined with simple procedures of chemical modifications of glycoproteins immobilized on ELISA plates or blots (desialylation by mild acid hydrolysis, Smith degradation, β-elimination). These modifications are helpful in the determination of lectin and anti-carbohydrate antibody specificities, or in the characterization of glycoconjugates by means of lectins and antibodies.

Original language	English
Pages (from-to)	127-132
Number of pages	6
Journal	Advances in Experimental Medicine and Biology
Volume	491
DOIs	https://doi.org/10.1007/978-1-4615-1267-7_9
State	Published - 2001

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title = "Lectin and anti-carbohydrate antibody assays using chemically modified ligands",

abstract = "Microtiter plate assays and 'lectinoblotting' with the use of biotinylated lectins are sensitive and easy to perform methods that can be combined with simple procedures of chemical modifications of glycoproteins immobilized on ELISA plates or blots (desialylation by mild acid hydrolysis, Smith degradation, β-elimination). These modifications are helpful in the determination of lectin and anti-carbohydrate antibody specificities, or in the characterization of glycoconjugates by means of lectins and antibodies.",

author = "Maria Duk and Wu, \{Albert M.\} and Elwira Lisowska",

year = "2001",

doi = "10.1007/978-1-4615-1267-7\_9",

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volume = "491",

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journal = "Advances in Experimental Medicine and Biology",

issn = "0065-2598",

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T1 - Lectin and anti-carbohydrate antibody assays using chemically modified ligands

AU - Duk, Maria

AU - Wu, Albert M.

AU - Lisowska, Elwira

PY - 2001

Y1 - 2001

N2 - Microtiter plate assays and 'lectinoblotting' with the use of biotinylated lectins are sensitive and easy to perform methods that can be combined with simple procedures of chemical modifications of glycoproteins immobilized on ELISA plates or blots (desialylation by mild acid hydrolysis, Smith degradation, β-elimination). These modifications are helpful in the determination of lectin and anti-carbohydrate antibody specificities, or in the characterization of glycoconjugates by means of lectins and antibodies.

AB - Microtiter plate assays and 'lectinoblotting' with the use of biotinylated lectins are sensitive and easy to perform methods that can be combined with simple procedures of chemical modifications of glycoproteins immobilized on ELISA plates or blots (desialylation by mild acid hydrolysis, Smith degradation, β-elimination). These modifications are helpful in the determination of lectin and anti-carbohydrate antibody specificities, or in the characterization of glycoconjugates by means of lectins and antibodies.

UR - https://www.scopus.com/pages/publications/0034951477

U2 - 10.1007/978-1-4615-1267-7_9

DO - 10.1007/978-1-4615-1267-7_9

M3 - 文章

C2 - 14533794

AN - SCOPUS:0034951477

SN - 0065-2598

VL - 491

SP - 127

EP - 132

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Lectin and anti-carbohydrate antibody assays using chemically modified ligands

Abstract

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