Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells

Ming Han Yeh, Tzung Chieh Tsai, Han Peng Kuo, Nai Wen Chang, Miao Rong Lee, Jing Gung Chung, Ming Hsui Tsai, Jah Yao Liu*, Ming Ching Kao

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

9 Scopus citations

Abstract

Oral carcinoma is a serious public health problem and the leading cause of head and neck cancer mortality worldwide. Moreover, oral cancer patients often present symptoms at a late stage and show a high recurrence rate after treatment. Therefore, there is an urgent need to identify novel biomarkers for early diagnosis or clinical oral cancer therapy. In this study, we employed a subset of lentiviral short hairpin RNAs targeted against various kinases and phosphatases, designed by The RNAi Consortium, to screen systemically and in a high-throughput manner for potential growth regulators of oral cancer cells. The screen revealed a total of 50 candidate genes, for which more than 90% of growth inhibition in human oral squamous cancer HSC-3 cells was obtained. Furthermore, bioinformatic analysis of these candidate genes identified transforming growth factor-β receptor type II- and fms-related tyrosine kinase 3-related molecular pathways that are involved in NF-κB-mediated growth of HSC-3 cells. These candidate genes may be potential biomarkers for early diagnosis of oral cancer. In addition, these candidate genes represent potential targets for anticancer drug design helping to develop a personalized treatment to combat oral cancer.

Original languageEnglish
Pages (from-to)1221-1231
Number of pages11
JournalInternational Journal of Oncology
Volume39
Issue number5
DOIs
StatePublished - 11 2011
Externally publishedYes

Keywords

  • Biomarkers
  • High-throughput screening assays
  • Kinases
  • Oral cancer
  • Phosphoprotein phosphatases
  • RNA interference

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