Leukocyte-poor platelet-rich plasma and leukocyte-rich platelet-rich plasma promote myoblast proliferation through the upregulation of cyclin A, cdk1, and cdk2

Li Siou Chen, Chih Kuang Chen, Jong Hwei Su Pang, Li Ping Lin, Tung Yang Yu, Wen Chung Tsai*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations

Abstract

Muscle injuries are common among athletes and often treated with platelet-rich plasma (PRP). However, whether the leukocyte concentration affects the efficacy of PRP in treating muscle injuries remains unclear. This study investigated the effects of leukocyte-poor platelet-rich plasma (LP-PRP) and leukocyte-rich platelet-rich plasma (LR-PRP) on myoblast proliferation and the molecular mechanisms underlying these effects. Myoblasts were treated with 0.5% LP-PRP, 0.5% LR-PRP, 1% LP-PRP, or 1% LR-PRP for 24 h. The gene expression of the LP-PRP- and LR-PRP–treated myoblasts was determined using RNA sequencing analysis. Cell proliferation was evaluated using an bromodeoxyuridine (BrdU) assay, and cell cycle progression was assessed through flow cytometry. The expression of cyclin A, cyclin-dependent kinase 1 (cdk1), and cdk2 was examined using Western blotting. The expression of myoblast determination protein 1 (MyoD1) was examined through Western blotting and immunofluorescence staining. The LP-PRP and LR-PRP both promoted the proliferation of myoblasts and increased differential gene expression of myoblasts. Moreover, the LP-PRP and LR-PRP substantially upregulated the expression of cyclin A, cdk1, and cdk2. MyoD1 expression was induced in the LP-PRP and LR-PRP–treated myoblasts. Our results corroborate the finding that LP-PRP and LR-PRP have similar positive effects on myoblast proliferation and MyoD1 expression.

Original languageEnglish
Pages (from-to)32-42
Number of pages11
JournalJournal of Orthopaedic Research
Volume42
Issue number1
DOIs
StatePublished - 01 2024

Bibliographical note

© 2023 Orthopaedic Research Society.

Keywords

  • leukocyte
  • myoblast
  • platelet-rich plasma
  • proliferation
  • Leukocytes/physiology
  • CDC2 Protein Kinase/metabolism
  • Up-Regulation
  • Cell Proliferation
  • Myoblasts/physiology
  • Humans
  • Platelet-Rich Plasma/metabolism
  • Cyclin A/metabolism

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