TY - JOUR
T1 - Levels and values of circulating endothelial progenitor cells, soluble angiogenic factors, and mononuclear cell apoptosis in liver cirrhosis patients
AU - Chen, Chih Hung
AU - Chang, Li Teh
AU - Tung, Wei Chih
AU - Chen, Yung Lung
AU - Chang, Chia Lo
AU - Leu, Steve
AU - Sun, Cheuk Kwan
AU - Tsai, Tzu Hsien
AU - Tsai, I. Ting
AU - Chang, Hsueh Wen
AU - Yip, Hon Kan
PY - 2012
Y1 - 2012
N2 - Background: The roles of circulating endothelial progenitor cell (EPC) and mononuclear cell apoptosis (MCA) in liver cirrhosis (LC) patients are unknown. Moreover, vascular endothelial growth factor (VEGF) and stromal cell-derived factor (SDF)-1α are powerful endogenous substances enhancing EPC migration into circulation. We assessed the level and function of EPCs [CD31/CD34 (E 1), KDR/CD34 (E2), CXCR4/CD34 (E3)], levels of MCA, VEGF and SDF-1α in circulation of LC patients. Methods: Blood sample was prospectively collected once for assessing EPC level and function, MCA, and plasma levels of VEGF and SDF-1α using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, in 78 LC patients and 25 age- and gender-matched healthy controls. Results: Number of EPCs (E1, E 2, E3) was lower (all p<0.0001), whereas SDF-1α level and MCA were higher (p<0.001) in study patients compared with healthy controls. Number of EPCs (E2, E3) was higher but MCA was lower (all p<0.05) in Child's class A compared with Child's class B and C patients, although no difference in VEGF and SDF-1α levels were noted among these patients. Chronic hepatitis B and esophageal varices bleeding were independently, whereas chronic hepatitis C, elevated aspartate aminotransferase (AST), and decompensated LC were inversely and independently correlated with circulating EPC level (all p<0.03). Additionally, angiogenesis and transwell migratory ability of EPCs were reduced in LC patients than in controls (all p<0.001). Conclusion: The results of this study demonstrated that level, angiogenic capacity, and function of circulating EPCs were significantly reduced, whereas plasma levels of SDF-1α and circulating MCA were substantially enhanced in cirrhotic patients.
AB - Background: The roles of circulating endothelial progenitor cell (EPC) and mononuclear cell apoptosis (MCA) in liver cirrhosis (LC) patients are unknown. Moreover, vascular endothelial growth factor (VEGF) and stromal cell-derived factor (SDF)-1α are powerful endogenous substances enhancing EPC migration into circulation. We assessed the level and function of EPCs [CD31/CD34 (E 1), KDR/CD34 (E2), CXCR4/CD34 (E3)], levels of MCA, VEGF and SDF-1α in circulation of LC patients. Methods: Blood sample was prospectively collected once for assessing EPC level and function, MCA, and plasma levels of VEGF and SDF-1α using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, in 78 LC patients and 25 age- and gender-matched healthy controls. Results: Number of EPCs (E1, E 2, E3) was lower (all p<0.0001), whereas SDF-1α level and MCA were higher (p<0.001) in study patients compared with healthy controls. Number of EPCs (E2, E3) was higher but MCA was lower (all p<0.05) in Child's class A compared with Child's class B and C patients, although no difference in VEGF and SDF-1α levels were noted among these patients. Chronic hepatitis B and esophageal varices bleeding were independently, whereas chronic hepatitis C, elevated aspartate aminotransferase (AST), and decompensated LC were inversely and independently correlated with circulating EPC level (all p<0.03). Additionally, angiogenesis and transwell migratory ability of EPCs were reduced in LC patients than in controls (all p<0.001). Conclusion: The results of this study demonstrated that level, angiogenic capacity, and function of circulating EPCs were significantly reduced, whereas plasma levels of SDF-1α and circulating MCA were substantially enhanced in cirrhotic patients.
KW - Angiogenesis factors
KW - Cellular apoptosis
KW - Circulating endothelial progenitor cells
KW - Liver cirrhosis
UR - http://www.scopus.com/inward/record.url?scp=84864650839&partnerID=8YFLogxK
U2 - 10.1186/1423-0127-19-66
DO - 10.1186/1423-0127-19-66
M3 - 文章
C2 - 22809449
AN - SCOPUS:84864650839
SN - 1021-7770
VL - 19
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
IS - 1
M1 - 66
ER -