Lidocaine inhibits migration of tenocytes by downregulating focal adhesion kinase and paxillin phosphorylation

Hsiang Ning Chang, Chih Kuang Chen, Tung Yang Yu, Jong Hwei S. Pang, Chih Chin Hsu, Li Ping Lin, Wen Chung Tsai*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review


Lidocaine is the most frequently applied local infiltration anesthetic agent for treating tendinopathies. However, studies have discovered lidocaine to negatively affect tendon healing. In the current study, the molecular mechanisms and effects of lidocaine on tenocyte migration were evaluated. We treated tenocytes intrinsic to the Achilles tendons of Sprague–Dawley rats with lidocaine. The migration ability of cells was analyzed using electric cell-substrate impedance sensing (ECIS) and scratch wound assay. We then used a microscope to evaluate the cell spread. We assessed filamentous actin (F-actin) cytoskeleton formation through immunofluorescence staining. In addition, we used Western blot analysis to analyze the expression of phospho-focal adhesion kinase (FAK), FAK, phospho-paxillin, paxillin, and F-actin. We discovered that lidocaine had an inhibitory effect on the migration of tenocytes in the scratch wound assay and on the ECIS chip. Lidocaine treatment suppressed cell spreading and changed the cell morphology and F-actin distribution. Lidocaine reduced F-actin formation in the tenocyte during cell spreading; furthermore, it inhibited phospho-FAK, F-actin, and phospho-paxillin expression in the tenocytes. Our study revealed that lidocaine inhibits the spread and migration of tenocytes. The molecular mechanism potentially underlying this effect is downregulation of F-actin, phospho-FAK, and phospho-paxillin expression when cells are treated with lidocaine.

Original languageEnglish
Pages (from-to)985-992
Number of pages8
JournalJournal of Orthopaedic Research
Issue number5
StatePublished - 05 2024

Bibliographical note

© 2023 Orthopaedic Research Society.


  • cell migration
  • focal adhesion kinase
  • lidocaine
  • paxillin
  • tenocyte
  • Paxillin/drug effects
  • Phosphorylation
  • Focal Adhesion Protein-Tyrosine Kinases/drug effects
  • Rats
  • Actins/drug effects
  • Tenocytes/drug effects
  • Cell Adhesion
  • Rats, Sprague-Dawley
  • Lidocaine/pharmacology
  • Animals
  • Achilles Tendon/drug effects
  • Cell Movement


Dive into the research topics of 'Lidocaine inhibits migration of tenocytes by downregulating focal adhesion kinase and paxillin phosphorylation'. Together they form a unique fingerprint.

Cite this