Light-emitting aeromonas and plesiomonas generated by transconjugating luxAB from Escherichia coli.

M. Z. Huang; J. H. Lee; S. T. Liu; W. C. Tsai

Light-emitting aeromonas and plesiomonas generated by transconjugating luxAB from Escherichia coli.

M. Z. Huang^*, J. H. Lee, S. T. Liu, W. C. Tsai

^*Corresponding author for this work

National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

Abstract

The transposon derivative has been placed on a transposition suicide vector to yield pDB30 in Escherichia coli WA803. A simple method, using a Tn5 derivative Tn5-Lux, has been successfully devised for the introduction and stable expression of the bioluminescence property in Pseudomonas sp., Agrobacterium sp., and Rhizobium sp. In this study, there was also successful mating between Escherichia coli WA803(pDB30) and strains of Acromonas hydrophila and Plesiomonas shigelloides. These bacteria emitted bioluminescence after they gained pDB30 by transconjugation.

Original language	English
Pages (from-to)	206-210
Number of pages	5
Journal	中華民國微生物及免疫學雜誌
Volume	27
Issue number	4
State	Published - 11 1994
Externally published	Yes

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Cite this

@article{22aeaeb63fcc42a4b799b75e3d3e1fbb,

title = "Light-emitting aeromonas and plesiomonas generated by transconjugating luxAB from Escherichia coli.",

abstract = "The transposon derivative has been placed on a transposition suicide vector to yield pDB30 in Escherichia coli WA803. A simple method, using a Tn5 derivative Tn5-Lux, has been successfully devised for the introduction and stable expression of the bioluminescence property in Pseudomonas sp., Agrobacterium sp., and Rhizobium sp. In this study, there was also successful mating between Escherichia coli WA803(pDB30) and strains of Acromonas hydrophila and Plesiomonas shigelloides. These bacteria emitted bioluminescence after they gained pDB30 by transconjugation.",

keywords = "單孢菌, 大腸桿菌, 轉移接合",

author = "Huang, {M. Z.} and Lee, {J. H.} and Liu, {S. T.} and Tsai, {W. C.}",

year = "1994",

month = nov,

language = "英语",

volume = "27",

pages = "206--210",

journal = "中華民國微生物及免疫學雜誌",

issn = "0253-2662",

publisher = "Lippincott Williams & Wilkins Asia",

number = "4",

}

TY - JOUR

T1 - Light-emitting aeromonas and plesiomonas generated by transconjugating luxAB from Escherichia coli.

AU - Huang, M. Z.

AU - Lee, J. H.

AU - Liu, S. T.

AU - Tsai, W. C.

PY - 1994/11

Y1 - 1994/11

N2 - The transposon derivative has been placed on a transposition suicide vector to yield pDB30 in Escherichia coli WA803. A simple method, using a Tn5 derivative Tn5-Lux, has been successfully devised for the introduction and stable expression of the bioluminescence property in Pseudomonas sp., Agrobacterium sp., and Rhizobium sp. In this study, there was also successful mating between Escherichia coli WA803(pDB30) and strains of Acromonas hydrophila and Plesiomonas shigelloides. These bacteria emitted bioluminescence after they gained pDB30 by transconjugation.

AB - The transposon derivative has been placed on a transposition suicide vector to yield pDB30 in Escherichia coli WA803. A simple method, using a Tn5 derivative Tn5-Lux, has been successfully devised for the introduction and stable expression of the bioluminescence property in Pseudomonas sp., Agrobacterium sp., and Rhizobium sp. In this study, there was also successful mating between Escherichia coli WA803(pDB30) and strains of Acromonas hydrophila and Plesiomonas shigelloides. These bacteria emitted bioluminescence after they gained pDB30 by transconjugation.

KW - 單孢菌

KW - 大腸桿菌

KW - 轉移接合

UR - http://www.scopus.com/inward/record.url?scp=0028535545&partnerID=8YFLogxK

M3 - 文章

C2 - 9747350

SN - 0253-2662

VL - 27

SP - 206

EP - 210

JO - 中華民國微生物及免疫學雜誌

JF - 中華民國微生物及免疫學雜誌

IS - 4

ER -

Light-emitting aeromonas and plesiomonas generated by transconjugating luxAB from Escherichia coli.

Abstract

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