TY - JOUR
T1 - Linked chromosome 16q13 chemokines, macrophage-derived chemokine, fractalkine, and thymus- and activation-regulated chemokine, are expressed in human atherosclerotic lesions
AU - Greaves, David R.
AU - Häkkinen, Tomi
AU - Lucas, Andrew D.
AU - Liddiard, Kate
AU - Jones, Emma
AU - Quinn, Carmel M.
AU - Senaratne, Jawaharlal
AU - Green, Fiona R.
AU - Tyson, Kerry
AU - Boyle, Joe
AU - Shanahan, Cathy
AU - Weissberg, Peter L.
AU - Gordon, Siamon
AU - Ylä-Hertualla, Seppo
PY - 2001
Y1 - 2001
N2 - Chemokines are important mediators of macrophage and T-cell recruitment in a number of inflammatory pathologies, and chemokines expressed in atherosclerotic lesions may play an important role in mononuclear cell recruitment and macrophage differentiation. We have analyzed the expression of the linked chromosome 16q13 genes that encode macrophage-derived chemokine (MDC/CCL22), thymus-and activation-regulated chemokine (TARC/ CCL 17), and the CX3C chemokine fractalkine (CX3CL1) in primary macrophages and human atherosclerotic lesions by reverse transcription-polymerase chain reaction and immunohistochemistry. We show that macrophage expression of the chemokines MDC, fractalkine, and TARC is upregulated by treatment with the Th2-type cytokines interleukin-4 and interleukin-13. High levels of MDC, TARC, and fractalkine mRNA expression are seen in some, but not all, human arteries with advanced atherosclerotic lesions. Immunohistochemistry shows that MDC, fractalkine, and TARC are expressed by a subset of macrophages within regions of plaques that contain plaque microvessels. We conclude that MDC, fractalkine, and TARC, which are chromosome 16q13 chemokines, could play a role in mononuclear cell recruitment into atherosclerotic lesions and influence the subsequent inflammatory response. Macrophage-expressed chemokines upregulated by interleukin-4 may be useful surrogate markers for the presence of Th2-type immune responses in human atherosclerotic lesions.
AB - Chemokines are important mediators of macrophage and T-cell recruitment in a number of inflammatory pathologies, and chemokines expressed in atherosclerotic lesions may play an important role in mononuclear cell recruitment and macrophage differentiation. We have analyzed the expression of the linked chromosome 16q13 genes that encode macrophage-derived chemokine (MDC/CCL22), thymus-and activation-regulated chemokine (TARC/ CCL 17), and the CX3C chemokine fractalkine (CX3CL1) in primary macrophages and human atherosclerotic lesions by reverse transcription-polymerase chain reaction and immunohistochemistry. We show that macrophage expression of the chemokines MDC, fractalkine, and TARC is upregulated by treatment with the Th2-type cytokines interleukin-4 and interleukin-13. High levels of MDC, TARC, and fractalkine mRNA expression are seen in some, but not all, human arteries with advanced atherosclerotic lesions. Immunohistochemistry shows that MDC, fractalkine, and TARC are expressed by a subset of macrophages within regions of plaques that contain plaque microvessels. We conclude that MDC, fractalkine, and TARC, which are chromosome 16q13 chemokines, could play a role in mononuclear cell recruitment into atherosclerotic lesions and influence the subsequent inflammatory response. Macrophage-expressed chemokines upregulated by interleukin-4 may be useful surrogate markers for the presence of Th2-type immune responses in human atherosclerotic lesions.
KW - Atherosclerosis
KW - Chemokines
KW - Macrophages
KW - Th2-type T cells
UR - https://www.scopus.com/pages/publications/0035715132
U2 - 10.1161/01.ATV.21.6.923
DO - 10.1161/01.ATV.21.6.923
M3 - 文章
C2 - 11397698
AN - SCOPUS:0035715132
SN - 1079-5642
VL - 21
SP - 923
EP - 929
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
IS - 6
ER -