Liquid Chromatographic Determination of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Inhibitors

Pei Ming Shen*, Ming Shi Shiao, Huey Ru Chung, Kuan Rong Lee, Yu Sheng Chao, Vincent M. Hunt

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

16 Scopus citations

Abstract

Reversed-phase high-performance liquid chromatography (RP-HPLC) was used as a tool to explore the retention behavior and separation of four 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, namely compactin, lovastatin, simvastatin, and pravastatin in their hydroxy acid and lactone forms. The contribution of C-6 and C-2′ methyl groups and lactonization to the molecular hydrophobicity among these four structurally related HMG-CoA reductase inhibitors were elucidated. Eight components (four lactones and four hydroxy acids) could be resolved by RP-HPLC with isocratic elution. In a binary mobile phase system of acetonitrile-water containing 0.5% acetic acid, the free hydroxy acids and corresponding lactone forms remained intact and were completely separated. This study demonstrated that RP-HPLC is suitable for simultaneous determination of active and prodrug forms of these HMG-CoA reductase inhibitors.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalJournal of the Chinese Chemical Society
Volume43
Issue number5
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Compactin
  • HMG-CoA reductase inhibitor
  • Lovastatin
  • Pravastatin
  • Reversed phase-HPLC
  • Simvastatin

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