Abstract
Reversed-phase high-performance liquid chromatography (RP-HPLC) was used as a tool to explore the retention behavior and separation of four 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, namely compactin, lovastatin, simvastatin, and pravastatin in their hydroxy acid and lactone forms. The contribution of C-6 and C-2′ methyl groups and lactonization to the molecular hydrophobicity among these four structurally related HMG-CoA reductase inhibitors were elucidated. Eight components (four lactones and four hydroxy acids) could be resolved by RP-HPLC with isocratic elution. In a binary mobile phase system of acetonitrile-water containing 0.5% acetic acid, the free hydroxy acids and corresponding lactone forms remained intact and were completely separated. This study demonstrated that RP-HPLC is suitable for simultaneous determination of active and prodrug forms of these HMG-CoA reductase inhibitors.
Original language | English |
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Pages (from-to) | 451-457 |
Number of pages | 7 |
Journal | Journal of the Chinese Chemical Society |
Volume | 43 |
Issue number | 5 |
DOIs | |
State | Published - 1996 |
Externally published | Yes |
Keywords
- Compactin
- HMG-CoA reductase inhibitor
- Lovastatin
- Pravastatin
- Reversed phase-HPLC
- Simvastatin