Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells

Kuo Shyang Jeng, Chi Juei Jeng, Wen Juei Jeng, I. Shyan Sheen, Shih Yun Li, Zih Hang Hung, Hsin I. Hsiau, Ming Che Yu, Chiung Fang Chang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations


Hepatocellular carcinoma (HCC) is a worldwide malignancy with poor prognosis. Liver progenitors or stem cells could be a potential therapy for HCC treatment since they migrate toward tumors. Rat liver epithelial (RLE) cells have both progenitor and stem cell-like properties. Therefore, our study elucidated the therapeutic effect of RLE cells in rat hepatoma cells. RLE cells were isolated from 10-day old rats and characterized for stem cell marker expression. RLE cells and rat hepatoma cells (H4-IIE-C3 cells) were co-cultured and divided into four groups with different ratios of RLE and hepatoma cells. Group A had only rat hepatoma cells as a control group. The ratios of rat hepatoma and RLE cells in group B, C and D were 5:1, 1:1 and 1:5, respectively. Effective inhibition of cell proliferation and migration was found in group D when compared to group A. There was a significant decrease in Bcl2 expression and increase in late apoptosis of rat hepatoma cells when adding more RLE cells. RLE cells reduced cell proliferation and migration of rat hepatoma cells. These results suggested that RLE cells could be used as a potential cell therapy.

Original languageEnglish
Pages (from-to)1622-1628
Number of pages7
JournalOncology Reports
Issue number3
StatePublished - 03 2016


  • Apoptosis
  • Bcl2
  • Cell therapy
  • Hepatocellular carcinoma
  • Rat liver epithelial cells


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