Long-acting beta 2 agonists suppress IP-10 expression in human bronchial epithelial cells

Jien Wen Chien*, Yu Te Chu, San Nan Yang, Chang Hung Kuo, Wei Li Wang, Po Lin Kuo, Yuh Jyh Jong, Chih Hsing Hung

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

5 Scopus citations


Background: Interferon-F-inducible protein (IP)-10 (CXCL10) is an important chemokine secreted by the airway epithelium that functions as a biomarker for virus-induced asthma. Long-acting beta 2 (β2) agonists (LABAs) are frequently used as inhaledmedication for asthma and chronic obstructive pulmonary disease. However, previous research failed to investigate the effects of LABAs on IP-10 in bronchial epithelial cells. Objective: To study the effects and signaling pathways of formoterol and salmeterol on polyriboinosinic polyribocytidylic acid (poly I:C)-induced IP-10 expression in BEAS-2B cells. Methods: BEAS-2B cellswere pretreatedwith formoterol and salmeterol for 2 hours before poly I:C stimulation. ICI 118551 β2 adrenoreceptor antagonist) or mitogen-activated protein kinase (MAPK) inhibitors were added 30 minutes before LABAs were added. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction were used to measure IP-10 protein and messenger RNA levels. Mitogen-activated protein kinase inhibitors and Western blotting were used to identify MAPK pathways, whereas bioassay revealed the MAPK functions. Results: Long-acting β2 agonists significantly down-regulated the poly I:C-induced IP-10 protein and messenger RNA expression in BEAS-2B cells. ICI 118551 reversed this effect. Forskolin, a cyclic adenosine monophosphate activator, produced a similar inhibitory effect. Western blotting showed that formoterol suppressed poly I:C-induced IP-10 expression via the c-Jun N-terminal kinaseYc-Jun pathway. Conclusion: Long-acting β2 agonists down-regulate poly I:C-induced IP-10 expression in BEAS-2B cells via the β2 adrenoreceptorYcyclic adenosine monophosphate and c-Jun N-terminal kinase/c-Jun pathways. Long-acting β2 agonists also inhibit IP-10 production in bronchial epithelial cells and may prolong viral elimination.

Original languageEnglish
Pages (from-to)1048-1053
Number of pages6
JournalJournal of Investigative Medicine
Issue number7
StatePublished - 10 2012
Externally publishedYes


  • Bronchial epithelial cell
  • Chemokines
  • Long-acting beta 2 agonist
  • MAPKs
  • Virus


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