Low expression of pRB predicts disease relapse in early glottic cancer treated with transoral laser microsurgery

Li Ang Lee*, Tuan Jen Fang, Hsueh Yu Li, Chung Guei Huang, Tse Ching Chen, Chun Ta Liao, Chung Jan Kang, Kai Ping Chang, Tzu Chen Yen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations


Objectives/Hypothesis: To elucidate the associations among the immunohistochemical expression of tumor markers, clinicopathological variables, and disease-free survival (DFS) in patients with early-stage glottic squamous cell carcinoma (SCC) who underwent transoral laser microsurgery (TLM) as the primary treatment. Study Design: Retrospective chart review. Methods: The records of consecutive patients with Tis‒T2N0 glottic SCC who underwent TLM between August 1, 2012 and October 31, 2015 were reviewed. Expression of Bcl-2, pRB, p16INK4A, p53, c-Myc, E-cadherin, and EGFR was examined using tissue microarrays containing tumor specimens through immunohistochemistry. Three-year DFS rates were calculated. Results: A total of 65 consecutive patients were identified, of which 28 were excluded due to insufficient tissue (n = 22) and low biomarker quality (n = 6). Therefore, 37 patients with complete records were included. The included patients were significantly older and had a more advanced type of cordectomy than did the excluded patients (P =.015 and.009, respectively). According to the findings of univariate analysis, age, betel quid chewing, type of cordectomy, BCL-2 expression, and pRB expression significantly predicted 3-year DFS. According to the findings of multivariate analysis, age (adjusted hazard ratio: 0.94, 95% CI: 0.88-1.00), betel quid chewing (adjusted hazard ratio: 5.07, 95% CI: 1.32-19.44), and pRB expression (adjusted hazard ratio: 0.02, 95% CI: 0.00-0.28) were independent predictors of 3-year DFS. Conclusions: Low pRB expression is a potential biomarker for predicting disease relapse after primary TLM for early-stage glottic SCC and may help to identify high-risk patients who can subsequently undergo intensive management. Level of Evidence: 4. Laryngoscope, 129:E220–E226, 2019.

Original languageEnglish
Pages (from-to)E220-E226
Issue number6
StatePublished - 06 2019

Bibliographical note

Publisher Copyright:
© 2018 The American Laryngological, Rhinological and Otological Society, Inc.


  • Disease-free survival
  • early-stage glottic squamous cell carcinoma
  • immunohistochemistry
  • pRB
  • tissue microarray
  • transoral laser microsurgery


Dive into the research topics of 'Low expression of pRB predicts disease relapse in early glottic cancer treated with transoral laser microsurgery'. Together they form a unique fingerprint.

Cite this