Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Yi Ju Liu; Da Jeng Yao; Hwan You Chang; Chien Ming Liu; Chih Chen

doi:10.1016/j.bios.2008.06.019

Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Yi Ju Liu, Da Jeng Yao^*, Hwan You Chang, Chien Ming Liu, Chih Chen

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

54 Scopus citations

Abstract

This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

Original language	English
Pages (from-to)	558-565
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2008.06.019
State	Published - 01 12 2008
Externally published	Yes

Keywords

Biotin-streptavidin
Biotinlyted linker
DNA detection
Magnetic bead
Quantum dots

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10.1016/j.bios.2008.06.019

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title = "Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7",

abstract = "This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.",

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AU - Liu, Yi Ju

AU - Yao, Da Jeng

AU - Chang, Hwan You

AU - Liu, Chien Ming

AU - Chen, Chih

PY - 2008/12/1

Y1 - 2008/12/1

N2 - This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

AB - This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

KW - Biotin-streptavidin

KW - Biotinlyted linker

KW - DNA detection

KW - Magnetic bead

KW - Quantum dots

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AN - SCOPUS:53049105228

SN - 0956-5663

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IS - 4

ER -

Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

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Keywords

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