Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Yi Ju Liu; Da Jeng Yao; Hwan You Chang; Chien Ming Liu; Chih Chen

doi:10.1016/j.bios.2008.06.019

Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Yi Ju Liu
, Da Jeng Yao^*
, Hwan You Chang
, Chien Ming Liu
, Chih Chen

^*Corresponding author for this work

National Tsing Hua University
National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

59 Scopus citations

Abstract

This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

Original language	English
Pages (from-to)	558-565
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2008.06.019
State	Published - 01 12 2008
Externally published	Yes

Keywords

Biotin-streptavidin
Biotinlyted linker
DNA detection
Magnetic bead
Quantum dots

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10.1016/j.bios.2008.06.019

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title = "Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7",

abstract = "This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.",

keywords = "Biotin-streptavidin, Biotinlyted linker, DNA detection, Magnetic bead, Quantum dots",

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doi = "10.1016/j.bios.2008.06.019",

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AU - Liu, Yi Ju

AU - Yao, Da Jeng

AU - Chang, Hwan You

AU - Liu, Chien Ming

AU - Chen, Chih

PY - 2008/12/1

Y1 - 2008/12/1

N2 - This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

AB - This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

KW - Biotin-streptavidin

KW - Biotinlyted linker

KW - DNA detection

KW - Magnetic bead

KW - Quantum dots

UR - https://www.scopus.com/pages/publications/53049105228

U2 - 10.1016/j.bios.2008.06.019

DO - 10.1016/j.bios.2008.06.019

M3 - 文章

C2 - 18644710

AN - SCOPUS:53049105228

SN - 0956-5663

VL - 24

SP - 558

EP - 565

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 4

ER -

Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Abstract

Keywords

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